dc.contributor.author | Heritier, C | |
dc.contributor.author | Nordmann, P | |
dc.contributor.author | Tolun, V | |
dc.contributor.author | Poirel, L | |
dc.date.accessioned | 2021-03-02T22:43:32Z | |
dc.date.available | 2021-03-02T22:43:32Z | |
dc.date.issued | 2004 | |
dc.identifier.citation | Poirel L., Heritier C., Tolun V., Nordmann P., "Emergence of oxacillinase-mediated resistance to imipenem in Klebsiella pneumoniae", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, cilt.48, sa.1, ss.15-22, 2004 | |
dc.identifier.issn | 0066-4804 | |
dc.identifier.other | av_0e50c901-97b8-4ee3-b25a-73ecfe135433 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/15204 | |
dc.identifier.uri | https://doi.org/10.1128/aac.48.1.15-22.2004 | |
dc.description.abstract | Klebsiella pneumoniae strain 11978 was isolated in Turkey in 2001 and was found to be resistant to all beta-lactams, including carbapenems. Cloning and expression in Escherichia coli identified five beta-lactamases, including two novel oxacillinases. The beta-lactamase OXA-48 hydrolyzed imipenem at a high level and was remotely related (less than 46% amino acid identity) to the other oxacillinases. It hydrolyzed penicillins and imipenem but not expanded-spectrum cephalosporins. The bla(OXA-48) gene was plasmid encoded and not associated with an integron, in contrast to most of the oxacillinase genes. An insertion sequence, IS1999, was found immediately upstream of bla(OXA-48). Another plasmid that encoded a second oxacillinase gene, bla(OXA-47), located inside a class 1 integron was identified in K. pneumoniae 11978. OYA-47 had a narrow spectrum of hydrolysis activity and did not hydrolyze ceftazidime or imipenem, as is found for the beta-lactamase (OXA-1) to which it is related. In addition, beta-lactamases TEM-1 and SHV-2a were expressed from the same K. pneumoniae isolate. Analysis of the outer membrane proteins of this isolate revealed that it lacked a porin of ca. 36 kDa. Thus, the high-level resistance to beta-lactams of this clinical isolate resulted from peculiar beta-lactamases and modification of outer membrane proteins. | |
dc.language.iso | eng | |
dc.subject | Temel Bilimler | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Eczacılık | |
dc.subject | Temel Eczacılık Bilimleri | |
dc.subject | Farmakoloji ve Toksikoloji | |
dc.subject | FARMAKOLOJİ VE ECZACILIK | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Mikrobiyoloji | |
dc.title | Emergence of oxacillinase-mediated resistance to imipenem in Klebsiella pneumoniae | |
dc.type | Makale | |
dc.relation.journal | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | |
dc.contributor.department | , , | |
dc.identifier.volume | 48 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 15 | |
dc.identifier.endpage | 22 | |
dc.contributor.firstauthorID | 170610 | |