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dc.contributor.authorOzsoy, Serhat
dc.contributor.authorYilmaz, Huseyin
dc.contributor.authorTekelioglu, B. Kaan
dc.contributor.authorRicht, Juergen A.
dc.contributor.authorIqbal, Munir
dc.contributor.authorTURAN, Nuri
dc.contributor.authorOzsemir, Cemal
dc.contributor.authorYILMAZ, Aysun
dc.contributor.authorCizmecigil, Utku Y.
dc.contributor.authorAydin, Ozge
dc.contributor.authorBamac, Ozge Erdogan
dc.contributor.authorGurel, Aydin
dc.contributor.authorKutukcu, Ahmet
dc.contributor.authorOzsemir, Kubra
dc.contributor.authorTali, H. Emre
dc.contributor.authorTali, Besim H.
dc.contributor.authorYilmaz, Semaha G.
dc.contributor.authorYaramanoglu, Mehmetcan
dc.date.accessioned2021-12-10T09:41:09Z
dc.date.available2021-12-10T09:41:09Z
dc.date.issued2020
dc.identifier.citationTURAN N., Ozsemir C., YILMAZ A., Cizmecigil U. Y. , Aydin O., Bamac O. E. , Gurel A., Kutukcu A., Ozsemir K., Tali H. E. , et al., "Identification of Newcastle disease virus subgenotype VII.2 in wild birds in Turkey", BMC VETERINARY RESEARCH, cilt.16, sa.1, 2020
dc.identifier.issn1746-6148
dc.identifier.othervv_1032021
dc.identifier.otherav_0c5f69a3-9416-4a17-a217-8ed9428992f1
dc.identifier.urihttp://hdl.handle.net/20.500.12627/168251
dc.identifier.urihttps://avesis.istanbul.edu.tr/api/publication/0c5f69a3-9416-4a17-a217-8ed9428992f1/file
dc.identifier.urihttps://doi.org/10.1186/s12917-020-02503-3
dc.description.abstractBackground Newcastle disease viruses (NDVs) can spread across continents via migratory birds. Hence, we investigated the frequency of NDV in both non-migratory and birds migrating on the Black Sea-Mediterranean flyway, in Istanbul, Turkey. Birds were trapped using nets placed around the Kucukcekmece lake Avcilar, Istanbul, in spring seasons of 2016 and 2018. In total, 297 birds belonging to 42 different species were trapped, categorized according to species and sex, and flocked oropharyngeal swabs were collected. In addition, flocked swabs were also collected from 115 mallards caught by hunters around Edirne and from 207 birds which had been treated in the Veterinary Faculty of Istanbul university-Cerrahpasa. Tissue samples were taken from dead wild birds brought by public to Veterinary Faculty. A total of 619 flocked oropharyngeal swabs were pooled into 206 samples. RNA was extracted from swabs and tissue samples. Real-time RT-PCR prob. assay was used to detect NDV-RNA in samples. Results There was no amplification in real time RT-PCR in samples taken from wild birds caught by traps. However, amplification of NDV-F gene was observed in oropharyngeal swabs taken from 2 waterfowls (Common Moorhen and Mallard), and in tissue samples taken from 2 little owls and 1 common kestrel. Sequencing and phylogenetic analyses of these 5 samples for NDV-F gene showed great similarity with NDV subgenotype VII.2 viruses. Analysis also showed that there is a high similarity with the F gene sequences previously reported from Turkey in 2012 and as well as the sequences from neighbouring countries Bulgaria and Georgia and geographically close country such as Pakistan. Although the strains found in this study are closely related, there is a relatively small degree of molecular divergence within 543 bp of F gene of the Turkish NDV isolate and strains detected in Israel, Pakistan, Iran, United Arab Emirates and Belgium. Conclusions Our findings revealed the presence of subgenotype VII.2 of NDVs in wild birds in north west of Turkey and demonstrated some degree of molecular evolution when compared to the earlier NDV-VII.2 isolate in Turkey.
dc.language.isoeng
dc.subjectSmall Animals
dc.subjectHealth Sciences
dc.subjectTarımsal Bilimler
dc.subjectVeterinary (miscellaneous)
dc.subjectGeneral Veterinary
dc.subjectEquine
dc.subjectSağlık Bilimleri
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectBitki ve Hayvan Bilimleri
dc.subjectVETERİNERLİK BİLİMLERİ
dc.subjectVeteriner Bilimleri
dc.titleIdentification of Newcastle disease virus subgenotype VII.2 in wild birds in Turkey
dc.typeMakale
dc.relation.journalBMC VETERINARY RESEARCH
dc.contributor.departmentIstanbul University - Cerrahpasa , ,
dc.identifier.volume16
dc.identifier.issue1
dc.contributor.firstauthorID2740971


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