PROTECTIVE EFFECT OF DEXPANTHENOL AGAINST NEPHROTOXIC EFFECT OF AMIKACIN: AN EXPERIMENTAL STUDY

Abstract

Introduction and Aims:Aminoglycosides antibiotics accumulate at renal cortex and proximal tubule and causes acute tubular necrosis. Free oxygen radicals and oxidative stress are held responsible for the formation of nephrotoxic damage induced by aminoglycosides. Dexpanthenol is a derivative of pantothenic acid.Panthothenic acid and its derivatives play a major role in defense and repair mechanisms against inflammatory and oxidative stress at cellular level.Our aim in this study is to evaluate the protective effect of dexpanthenol against nephrotoxic effect of amikacinMethods:Thirty-two rats were randomly separated into four groups with eight in each(G1:Amikasin, G2: Dexpanthenol, G3: Amikasin+Dexpanthenol, G4:No treatment). Dexpanthenol was administered intraperitoneally in a dose of 500 mg/kg/day for the 14 days to the G2 and G3, while amikacin was administered intraperitoneally as a single daily dose of 1.2 g/kg on the 2nd, 3rd and 7th days to the G1 and G3).In biochemical parameters myeloperoxidase(MPO), total antioxidant status(TAS), total oxidant status(TOS), catalase(CAT), paraoxonase(PON), arylesterase(ARES), blood urea nitrogen (BUN) and creatinine (Cr) was studied in the blood samples.At the end of the 14th day, the rats were sacrified. Renal tissues were evaluated blindly by the pathologist.Results:TOS and Oxidative Stress Index(OSI) values of G3 were significantly lower than G1(p<0,05). Antioxidant biochemical parameters (MPO, TAS, CAT, PON, ARES) of G3 were found significantly higher than G1(p<0,05). BUN and creatinine values of G3 were found significantly lower than G1(p<0.05)(Table 1). In addition to those, while histopathologic changes such as tubular epithelial changes and interstitial edema were clearly observed in the G1, such findings were insignificant in the G3 (Figure 1-2).Conclusions:In our study, it was revealed with biochemical and histopathologic data that nephrotoxic effect of amikacin can be limited by dexpanthenol by being used together