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dc.contributor.authorJANNUZZI, Ayşe Tarbın
dc.date.accessioned2022-07-04T13:51:15Z
dc.date.available2022-07-04T13:51:15Z
dc.date.issued2022
dc.identifier.citationJANNUZZI A. T. , "Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes", Journal of Research in Pharmacy, cilt.26, sa.2, ss.354-361, 2022
dc.identifier.issn2630-6344
dc.identifier.othervv_1032021
dc.identifier.otherav_5cbfe4c6-2b76-40fa-b271-a60e90dd871b
dc.identifier.urihttp://hdl.handle.net/20.500.12627/182930
dc.identifier.urihttps://avesis.istanbul.edu.tr/api/publication/5cbfe4c6-2b76-40fa-b271-a60e90dd871b/file
dc.identifier.urihttps://doi.org/10.29228/jrp.133
dc.description.abstract© 2022 Marmara University Press.Eugenol is a phenolic compound isolated from clove essential oil. It is used in dentistry, fragrance, cosmetic, and food industries. According to the fragrance ingredient safety assessment report, eugenol does not cause phototoxic reactions and genotoxicity. However, its effect on UV-induced cytotoxicity and genotoxicity has not been well examined. Here in this study, cytotoxic and genotoxic effects of eugenol are investigated on UVA-induced damage using human keratinocyte cells (HaCaT). HaCaT cells were treated with increasing concentrations of eugenol (10-500 µM) for 1 hour and irradiated with 5-10-15 J/cm2 UVA. 24 hours later the neutral red uptake (NRU) assay was used to evaluate cytotoxicity. For genotoxicity assay cells were exposed to 1-10 µM eugenol for one hour and non-cytotoxic UVA irradiation doses (1, 2.5 J/cm2) were used. The alkaline comet assay was carried out immediately after the UVA irradiation to measure the genotoxic potential of eugenol. The cytotoxicity assay results indicate that eugenol caused a cytotoxic effect in a dose-dependent manner in HaCaT cells and increasing doses of UVA-irradiation enhanced the cytotoxic effect of eugenol. The alkaline comet assay results showed that eugenol causes DNA single-strand breaks and increasing doses of UVA-irradiation aggravates the genotoxic potential of eugenol. These data demonstrate that eugenol has cytotoxic and genotoxic potential and eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes.
dc.language.isoeng
dc.subjectLife Sciences
dc.subjectPharmacology (medical)
dc.subjectHealth Sciences
dc.subjectYaşam Bilimleri
dc.subjectGeneral Pharmacology, Toxicology and Pharmaceutics
dc.subjectTemel Bilimler
dc.subjectTemel Eczacılık Bilimleri
dc.subjectEczacılık
dc.subjectSağlık Bilimleri
dc.subjectFARMAKOLOJİ VE ECZACILIK
dc.subjectFarmakoloji ve Toksikoloji
dc.subjectYaşam Bilimleri (LIFE)
dc.titleEugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes
dc.typeMakale
dc.relation.journalJournal of Research in Pharmacy
dc.contributor.departmentİstanbul Üniversitesi , Eczacılık Fakültesi , Eczacılık Meslek Bilimleri Bölümü
dc.identifier.volume26
dc.identifier.issue2
dc.identifier.startpage354
dc.identifier.endpage361
dc.contributor.firstauthorID3422611


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