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dc.contributor.authorOnay Ucar, Evren
dc.contributor.authorAdiguzel, Zelal
dc.contributor.authorKiyga, Ezgi
dc.date.accessioned2023-02-21T09:39:45Z
dc.date.available2023-02-21T09:39:45Z
dc.date.issued2022
dc.identifier.citationKiyga E., Adiguzel Z., Onay Ucar E., "Temozolomide increases heat shock proteins in extracellular vesicles released from glioblastoma cells", MOLECULAR BIOLOGY REPORTS, cilt.49, sa.9, ss.8701-8713, 2022
dc.identifier.issn0301-4851
dc.identifier.othervv_1032021
dc.identifier.otherav_332565d1-0a21-4877-a26a-3f2105092cdd
dc.identifier.urihttp://hdl.handle.net/20.500.12627/187691
dc.identifier.urihttps://avesis.istanbul.edu.tr/api/publication/332565d1-0a21-4877-a26a-3f2105092cdd/file
dc.identifier.urihttps://doi.org/10.1007/s11033-022-07714-5
dc.description.abstractBackground Glioblastoma (GBM) is the most malignant and the fastest-progressing type of primary brain tumours. Temozolomide (TMZ) is a chemotherapeutic drug for the treatment of GBM. Extracellular vesicles (EVs) have been recently confirmed to have a substantial role in the GBM, and their contents released from GBM cells have been considered a target for treatment. The purpose of this study is to evaluate the impact of TMZ on heat shock proteins (HSPs) derived from EVs originated from GBM cell lines (U87-MG and LN229) and the significance of EVs in response to chemotherapy in GBM. Methods and results NTA, ELISA, and immunoblotting were used to characterization studies of EVs and results showed that U87-MG cells released many EVs compared to LN229 cells. The effect of TMZ treatments on HSPs expression levels were assessed with immunoblotting and was found to be led to increases in HSF-1, Hsp90, Hsp70, Hsp60 and Hsp27 expression in GBM cells and their EV contents, which these increases are related to therapeutic resistance. What is more, in Real-time PCR studies showing which signalling pathways might be associated with these increases, it was observed that TMZ triggered the expression of RAD51 and MDM2 genes in cells and EV contents. More strikingly, we discover a correlation between EV and parental cells in regard of mRNA and protein level in both cell lines as a result of TMZ treatment. Conclusions Our data suggest of EVs in the treatment of GBM may have potential biomarkers that can be used to investigate the treatment response.
dc.language.isoeng
dc.subjectCancer Research
dc.subjectMolecular Biology
dc.subjectDrug Discovery
dc.subjectAging
dc.subjectGeneral Biochemistry, Genetics and Molecular Biology
dc.subjectBiochemistry
dc.subjectStructural Biology
dc.subjectLife Sciences
dc.subjectBiochemistry, Genetics and Molecular Biology (miscellaneous)
dc.subjectClinical Biochemistry
dc.subjectTemel Bilimler
dc.subjectSitogenetik
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectYaşam Bilimleri
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.titleTemozolomide increases heat shock proteins in extracellular vesicles released from glioblastoma cells
dc.typeMakale
dc.relation.journalMOLECULAR BIOLOGY REPORTS
dc.contributor.departmentİstanbul Üniversitesi , Fen Fakültesi , Moleküler Biyoloji ve Genetik Bölümü
dc.identifier.volume49
dc.identifier.issue9
dc.identifier.startpage8701
dc.identifier.endpage8713
dc.contributor.firstauthorID3435007


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