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dc.contributor.authorZerdali, Esra
dc.contributor.authorOzaras, Resat
dc.contributor.authorTABAK, ÖMER FEHMİ
dc.contributor.authorSALTOĞLU, NEŞE
dc.contributor.authorMETE, BİLGÜL
dc.contributor.authorAkdogan, Ecem
dc.contributor.authorÖZPINAR, Esra
dc.date.accessioned2023-10-10T12:36:19Z
dc.date.available2023-10-10T12:36:19Z
dc.date.issued2023
dc.identifier.citationZerdali E., ÖZPINAR E., Akdogan E., METE B., SALTOĞLU N., TABAK Ö. F., Ozaras R., "The Effect of the Administration of Interferon and Steroids on Regulatory T-cells in the Liver, Spleen, and Bone Marrow of Mice", VIRAL HEPATIT DERGISI-VIRAL HEPATITIS JOURNAL, cilt.28, sa.3, ss.110-116, 2023
dc.identifier.issn1307-9441
dc.identifier.otherav_2426d428-2c79-4838-a011-fdd8aea428a0
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/190192
dc.identifier.urihttps://avesis.istanbul.edu.tr/api/publication/2426d428-2c79-4838-a011-fdd8aea428a0/file
dc.identifier.urihttps://doi.org/10.4274/vhd.galenos.2023.2023-4-3
dc.description.abstractObjectives: Regulatory T-cells (T-regs) maintain immune tolerance by affecting other cells of the immune system. They play an important role in autoimmune diseases and the prevention of graft rejection. Steroids suppress the immune system, especially inhibiting cytokine secretion of T-lymphocytes, initiation of the cell -mediated immune response, and stimulation of T-regs. Interferons (IFN) also have immunomodulatory, antiviral, and anti-proliferative effects. They activate macrophages and cytotoxic T-cells and stimulate the differentiation of T-regs. The aim of this study was to evaluate the effects of IFN and steroids on T-regs in the liver, spleen, and bone marrow in a mouse model, and to determine if they exert their immunosuppresive/immunomodulatory effects through T-regs.Materials and Methods: A total of 24 mice were randomly separated into 3 groups and administered an intraperitoneal injection for five days. The control group received 0.1 mL saline every day, the IFN group received IFN-alpha-2b 20,000 IU on the first, third, and fifth days, and only 0.1 mL saline on the other days, and the steroids group received 5 mg/kg dexamethasone in 0.1 mL saline every day. Two days after the end of therapy, each mouse was anesthetized, the portal vein was explored via laparotomy, and 5 mL bovine serum albumin (BSA) was administered through the portal vein. The inferior vena cava was cut to allow BSA perfusion of the liver, and then the mice were sacrificed. The liver, spleen, and bone marrow were removed for analysis. T-regs were identified and counted using flow cytometry.Results: The flow cytometry count results showed no significant difference between the IFN, steroid, and control groups. Conclusion: IFN and steroid use do not seem to affect the quantity of T-regs.
dc.language.isoeng
dc.subjectDeğerlendirme ve Teşhis
dc.subjectTemel Bilgi ve Beceriler
dc.subjectPatofizyoloji
dc.subjectTIP, GENEL & DAHİLİ
dc.subjectSağlık Bilimleri
dc.subjectKlinik Tıp
dc.subjectGenel Tıp
dc.subjectTıp (çeşitli)
dc.subjectAile Sağlığı
dc.subjectDahiliye
dc.subjectTıp
dc.subjectTemel Tıp Bilimleri
dc.subjectGenel Sağlık Meslekleri
dc.subjectKlinik Tıp (MED)
dc.titleThe Effect of the Administration of Interferon and Steroids on Regulatory T-cells in the Liver, Spleen, and Bone Marrow of Mice
dc.typeMakale
dc.relation.journalVIRAL HEPATIT DERGISI-VIRAL HEPATITIS JOURNAL
dc.contributor.departmentUniv Hlth Sci Turkey , ,
dc.identifier.volume28
dc.identifier.issue3
dc.identifier.startpage110
dc.identifier.endpage116
dc.contributor.firstauthorID4315635


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