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dc.contributor.authorDENİZLİ, ADİL
dc.contributor.authorÇAĞLAR, Senem
dc.contributor.authorANDAÇ, AYŞE MÜGE
dc.contributor.authorAndac, Cenk A.
dc.date.accessioned2023-10-10T12:55:50Z
dc.date.available2023-10-10T12:55:50Z
dc.date.issued2023
dc.identifier.citationAndac C. A., ÇAĞLAR S., DENİZLİ A., ANDAÇ A. M., "Isothermal titration calorimetry binding properties of Cibacron Blue F3GA in complex with human serum albumin", Journal of Molecular Recognition, cilt.36, sa.8, 2023
dc.identifier.issn0952-3499
dc.identifier.othervv_1032021
dc.identifier.otherav_2894e1d7-051c-4c07-8bff-7d9f556caad7
dc.identifier.urihttp://hdl.handle.net/20.500.12627/190330
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85161455878&origin=inward
dc.identifier.urihttps://doi.org/10.1002/jmr.3040
dc.description.abstractBinding interactions between Cibacron Blue-F3GA (CB-F3GA) and human serum albumin (HSA, at physiologically ten-fold lower concentration) was studied by isothermal titration calorimetry (ITC) and in-silico docking computations. ITC experiments revealed two separate binding sites on HSA with different binding affinities for CB-F3GA. The high-affinity binding site (PBS-II) on HSA binds CB-F3GA at nanomolar scale (KD1 = 118 ± 107 nM) with favorable binding enthalpy (ΔHo1 = − 6.47 ± 0.44 kcal/mol) and entropy (−TΔSo1 = −2.98 kcal/mol) energies. CB-F3GA binds to the low-affinity binding site (PBS-I) at μM scale (KD2 = 31.20 ± 18.40 μM) with favorable binding enthalpy (ΔHo1 = − 5.03 ± 3.86 × 10−2 kcal/mol) and entropy (−TΔSo1 = −1.12 kcal/mol) energies. ITC binding data strongly suggest that CB-F3GA binding to PBS-II site increases the formation of dimeric-HSA clusters (N1 = 2.43 ± 0.50), while binding to PBS-I leads to tetrameric-HSA clusters (N2 = 4.61 ± 0.90). These results suggest that a higher degree of HSA aggregation upon drug binding may be expected under physiological conditions, a notion that should be further investigated for the delivery and toxicity of drug−HSA interactions.
dc.language.isoeng
dc.subjectTemel Bilimler
dc.subjectYapısal Biyoloji
dc.subjectMoleküler Biyoloji
dc.subjectSitogenetik
dc.subjectYaşam Bilimleri
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectYaşam Bilimleri (LIFE)
dc.titleIsothermal titration calorimetry binding properties of Cibacron Blue F3GA in complex with human serum albumin
dc.typeMakale
dc.relation.journalJournal of Molecular Recognition
dc.contributor.departmentYeditepe University , ,
dc.identifier.volume36
dc.identifier.issue8
dc.contributor.firstauthorID4288779


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