Downregulation of NPRL2 in Colon Cancer

Abstract

Downregulation of NPRL2 in Colon Cancer

N. Buyru,1 İ.Hatemi2, İ.Aydin3, B.Yogurtcu1

1 Istanbul University Cerrahpasa Medicine Faculty Medical Biology Department

2 Istanbul University Cerrahpasa Medicine Faculty Department of Gastroenterology

3 Istanbul Training and Research Hospital Department of General Surgery

Colorectal cancer is a major cause of cancer related death all around the world. Genetic and epigenetic factors affecting DNA methylation and gene expression are known to be involved in the development of CRC, but full range of genetic alterations and many key genes involved in the pathogenesis of CRC remain to be identified.

NPRL2 is one of the candidate tumor suppressor genes identified in the human chromosome 3p21.3 region, which contains genetic abnormalities are frequently found in the early stages of various cancers. The aim of this study to evaluate the role of NPRL2 gene in the pathogenesis of colorectal cancer. Therefore we investigated NPRL2 mRNA expression in 55 normal and tumor colon tissue samples using quantitative real-time reverse transcription polymerase chain reaction analysis and the correlation between NPRL2 expression and clinicopathologic parameters.

RNA expression patterns showed that the decreased NPRL2 expression was observed in 46.15% of the patients. Unaffected NPRL2 expression was detected in 23.07 of patients. There was not a significant correlation between NPRL2 expression and in a clinicopathological parameters.

Our results suggest that the expression of NPRL2 may contribute to the progression of colon cancer however further studies are required to elucidated the role of NPRL2 in colon tumorigenesis.