dc.contributor.author | Külekçi, Mehmet | |
dc.contributor.author | Topcuoglu, Nursen | |
dc.contributor.author | Ustek, Duran | |
dc.contributor.author | Kulekci, Guven | |
dc.contributor.author | Paltura, Ceki | |
dc.date.accessioned | 2021-03-05T09:17:52Z | |
dc.date.available | 2021-03-05T09:17:52Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Topcuoglu N., Paltura C., Külekçi M., Ustek D., Kulekci G., "REAL-TIME POLYMERASE CHAIN REACTION VERSUS CONVENTIONAL PCR: A COMPARISON BETWEEN TWO METHODS FOR THE DETECTION OF FUSOBACTERIUM NUCLEATUM IN SALIVA, NASOPHARYNGEAL SECRETION AND MIDDLE EAR EFFUSION SAMPLES", BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.27, ss.3825-3828, 2013 | |
dc.identifier.issn | 1310-2818 | |
dc.identifier.other | av_9d2b8548-32af-4d24-99db-69cd108ae555 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/105585 | |
dc.identifier.uri | https://doi.org/10.5504/bbeq.2013.0022 | |
dc.description.abstract | Oral and middle ear infections are biofilm related infections. Fusobacterium nucleatum is considered to be a key species in dental biofilm formation. The aim of this study was to compare conventional and real-time PCR, for the detection of F. nucleatum in saliva, nasopharyngeal secretion and middle ear effusion samples. Sixty samples were collected from 20 patients with otitis media with effusion. Species-specific 165 rRNA primers were used for conventional PCR and species-specific primers and TaqMan probes were used for real-time PCR analysis. Real-time PCR had a better performance than conventional PCR in detecting bacteria in the analyzed samples. The tested real-time PCR assay can identify) and identify) F nucleatum in biofilm samples with a high degree of sensitivity when compared with conventional PCR techniques. | |
dc.language.iso | eng | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Biyoteknoloji | |
dc.subject | BİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ | |
dc.subject | Mikrobiyoloji | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Temel Bilimler | |
dc.title | REAL-TIME POLYMERASE CHAIN REACTION VERSUS CONVENTIONAL PCR: A COMPARISON BETWEEN TWO METHODS FOR THE DETECTION OF FUSOBACTERIUM NUCLEATUM IN SALIVA, NASOPHARYNGEAL SECRETION AND MIDDLE EAR EFFUSION SAMPLES | |
dc.type | Makale | |
dc.relation.journal | BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | |
dc.contributor.department | Istanbul Gaziosmanpasa Taksim Training & Research Hospital , , | |
dc.identifier.volume | 27 | |
dc.identifier.issue | 3 | |
dc.identifier.startpage | 3825 | |
dc.identifier.endpage | 3828 | |
dc.contributor.firstauthorID | 74695 | |