dc.contributor.author | Guzel, Elif | |
dc.contributor.author | Ozhan, Gül | |
dc.contributor.author | Abudayyak, Mahmoud | |
dc.date.accessioned | 2021-03-05T09:49:44Z | |
dc.date.available | 2021-03-05T09:49:44Z | |
dc.identifier.citation | Abudayyak M., Guzel E., Ozhan G., "Nickel oxide nanoparticles are highly toxic to SH-SY5Y neuronal cells", NEUROCHEMISTRY INTERNATIONAL, cilt.108, ss.7-14, 2017 | |
dc.identifier.issn | 0197-0186 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_9fdcc2ff-f1a4-46bd-9be8-5686524b7941 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/107263 | |
dc.identifier.uri | https://doi.org/10.1016/j.neuint.2017.01.017 | |
dc.description.abstract | Nickel oxide nanoparticles (NiO-NPs) are used in many industrial sectors including printing inks, ceramics and catalysts, and electrical and electronics industry because of their magnetic and optical properties. However, there has been still a serious lack of information about their toxicity at the cellular and molecular levels on nervous system. For that, we aimed to investigate the in vitro toxic potentials of NiO-NPs in neuronal (SH-SY5Y) cells. The particle characterisation, cellular uptake and morphological changes were determined using Transmission Electron Microscopy, dynamic light scattering and Inductively Coupled Plasma-Mass Spectrometry. Then, the cytotoxicity was evaluated by MTT and neutral red uptake assays, the genotoxicity by comet assay, the oxidative potentials by the determination of malondialdehyde, 8-hydroxy deoxyguanosine, protein carbonyl, and glutathione levels with Enzyme-Linked Immune Sorbent Assays, and the apoptotic potentials by Annexin V-FITC apoptosis detection assay with propidium iodide. According to the results, it was observed that NiO-NPs (15.0 nm +/- 4.2 -38.1 nm); (i) were taken up by the cells in concentration dependent manner, (ii) caused 50% inhibition in cell viability at >= 229.34 mu g/mL, (iii) induced some morphological changes, (iv) induced dose dependent DNA damage (3.2-11.0 fold) and apoptosis (80-99%), (v) significantly induced oxidative damage. In conclusion, our results support the hypothesis that NiO-NPs affect human health especially neuronal system negatively and should raise the concern about the safety associated with their applications in consumer products. (C) 2017 Elsevier Ltd. All rights reserved. | |
dc.language.iso | eng | |
dc.subject | Sitogenetik | |
dc.subject | Temel Bilimler | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Sinirbilim ve Davranış | |
dc.subject | NEUROSCIENCES | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | BİYOKİMYA VE MOLEKÜLER BİYOLOJİ | |
dc.title | Nickel oxide nanoparticles are highly toxic to SH-SY5Y neuronal cells | |
dc.type | Makale | |
dc.relation.journal | NEUROCHEMISTRY INTERNATIONAL | |
dc.contributor.department | İstanbul Üniversitesi , , | |
dc.identifier.volume | 108 | |
dc.identifier.startpage | 7 | |
dc.identifier.endpage | 14 | |
dc.contributor.firstauthorID | 65563 | |