Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa
Date
2013Author
Linhart, Otomar
Dzyuba, Borys
Cosson, Jacky
Bondarenko, Olga
Rodina, Marek
Gela, David
Bondarenko, Volodymir
Yamaner, GÜNEŞ
Shaliutina, Anna
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Post-thaw motility rate, curvilinear velocity (VCL), and fertilizing ability of carp spermatozoa can be improved by short-term treatment with moderately hypotonic media prior to freezing. Before cryopreservation, carp sperm samples were treated with NaCl solutions of differing osmolalities, ranging from 100 to 300 mOsm kg(-1) for 10 s, after which final osmolality was adjusted to 300 mOsm kg(-1). The resulting sperm suspension was diluted 1:1 with cryoprotective medium and frozen using conventional techniques. Control samples were treated in the same way, without the pre-dilution step. Post-thaw motility rate in samples pretreated with 200 mOsm kg(1) NaCl was significantly higher (44 +/- 10%) than in controls (21 +/- 15%) and samples pretreated with 100 mOsm kg(-1) (25 +/- 15%) and 300 mOsm kg(-1) (25 +/- 12%) NaCl. Significantly higher mean VCL were observed in samples pretreated with 100, 150, and 200 mOsm kg(-1) (119 +/- 24, 118 +/- 22, and 115 +/- 32 mu m s(-1), respectively) compared to controls (92 27 mu m s-1). Fertilization rate of frozen-thawed sperm treated with 200 mOsm kg(-1) solution of 2 M NaCl was significantly higher (25 +/- 18%) than that of sperm treated with 300 mOsm kg(-1) NaCl solutions (12 +/- 7%) and the control (9 +/- 6%). (C) 2012 Elsevier Inc. All rights reserved.
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