Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection

Date
2018
Author
Ayaz, Gulsel
Saribas, Suat
Yuksel, Pelin
Dinc, Harika O.
Toprak, Sezer
Demirci, Mehmet
ZIVER, Tevhide
Ozer, Nigar
CAGLAR, Emel
ORTAKOYLU, Gonenc
Tokman, Hrisi B.
UYSAL, ÖMER
Kocazeybek, Bekir
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Abstract
Background: Several nucleic acid amplification techniques (IS6110, 16S rRNA, and 85B mRNA) were developed for the rapid, direct detection of Mycobacterium tuberculosis. We aimed to assess the diagnostic performance of 85B mRNA-based RT-qPCR by comparing with the real-time PCR COBAS TagMan MTB Kit while using the BACTEC MGIT 960 method as the gold standard.
URI
http://hdl.handle.net/20.500.12627/117417
https://doi.org/10.1016/j.jiph.2018.02.002
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