dc.contributor.author | SARIHAN, MEHMET | |
dc.contributor.author | AKDOĞAN, MEHMET | |
dc.contributor.author | Onen, Hacer Ilke | |
dc.contributor.author | Guney Eskiler, Gamze | |
dc.contributor.author | KALAYCI YİĞİN, Aysel | |
dc.contributor.author | Deveci Ozkan, Asuman | |
dc.contributor.author | KALELİ, SÜLEYMAN | |
dc.date.accessioned | 2021-03-05T13:43:15Z | |
dc.date.available | 2021-03-05T13:43:15Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Deveci Ozkan A., KALELİ S., Onen H. I. , SARIHAN M., Guney Eskiler G., KALAYCI YİĞİN A., AKDOĞAN M., "Anti-inflammatory effects of nobiletin on TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways in prostate cancer cells", IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, cilt.42, ss.93-100, 2020 | |
dc.identifier.issn | 0892-3973 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_b3906acc-0abb-4e72-b312-65e50c6a371b | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/119603 | |
dc.identifier.uri | https://doi.org/10.1080/08923973.2020.1725040 | |
dc.description.abstract | Background: Toll-like receptors (TLRs) are often expressed in natural immune cells as well as in tumor cells. TLR4 exhibits both tumor promoting and tumor-suppressing roles and higher TLR9 expression is an important marker of poor prognosis in prostate cancer (PCa). Nobiletin (NOB) is an O-methylated flavonoid and NOB has been proven to have anti-cancer effect in PCa cells. However, there is no study in the literature investigating the potential anti-inflammatory effects of NOB on the TLR signaling pathways in cancer. Therefore, we aimed to explore the potential anti-inflammatory effects of NOB on the TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways in different types of PCa cell lines, for the first time. Material and methods: In the current study, the cytotoxic effect of NOB PC-3 (hormone-independent and metastatic) and LNCaP cells (hormone-dependent) was evaluated by WST-1 assay. Furthermore, the inhibitory effects of NOB on TLR4/TRIF/IRF3 and TLR9/IRF7signaling pathway were determined by RT-PCR, western blotting and ELISA analysis. Results: NOB demonstrated an inhibitory effect on PCa cell growth and LNCaP cells were more sensitive to NOB than PC-3 cells due to androjen receptor status. Furthermore, NOB alone could suppress TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways through the downregulation of their associated pathways (mRNA and related protein levels) and the release of IFN-alpha and IFN-beta compared to LPS or CpG-ODN stimulated PCa cells. Conclusions: NOB potentially inhibited TLR4 and TL9-dependent signaling pathway in PCa cells. However, the efficacy of NOB was different in PCa cells due to the hormone status and aggressive features. | |
dc.language.iso | eng | |
dc.subject | Meslek Bilimleri | |
dc.subject | Farmasötik Toksikoloji | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Temel Bilimler | |
dc.subject | TOKSİKOLOJİ | |
dc.subject | Eczacılık | |
dc.subject | Temel Eczacılık Bilimleri | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Farmakoloji ve Toksikoloji | |
dc.subject | FARMAKOLOJİ VE ECZACILIK | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | İmmünoloji | |
dc.title | Anti-inflammatory effects of nobiletin on TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways in prostate cancer cells | |
dc.type | Makale | |
dc.relation.journal | IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY | |
dc.contributor.department | Sakarya Üniversitesi , , | |
dc.identifier.volume | 42 | |
dc.identifier.issue | 2 | |
dc.identifier.startpage | 93 | |
dc.identifier.endpage | 100 | |
dc.contributor.firstauthorID | 2277549 | |