Characterization of two triterpenes and a steroid from the cultured roots of Salvia amplexicaulis

Abstract

Callus, cell and root culture systems of Salvia amplexicaulis were established to study its secondary metabolites. The roots of Salvia amplexicaulis cultured in Schenk-Hildebrand (SH) medium supplemented with 1 mg l(-1) Indol butyric acid have shown hairy root characteristics. Root cultures produced two triterpenes oleanolic and ursolic acids and a steroid beta-sitosterol in SH medium supplemented with 1 mg l(-1) IBA.