Relationship Between Genomic Damage and Clinical Features in Dialysis Patients

Abstract

Patients with end-stage renal disease display enhanced genomic damage. We investigated the presence of genomic damage in the peripheral lymphocytes by using the micronucleus (MN) test and the factors associated with the MN frequency in hemodialysis (HD) and peritoneal dialysis (PD) patients. We studied 121 dialysis patients (60 HD and 61 PD) and 129 age-and gender-matched healthy controls. The MN analysis, used as a biomarker of chromosomal/DNA damage, was performed in peripheral lymphocytes by the cytokinesis-block method. Univariate analysis showed a significantly higher MN frequency in all patients in comparison with the controls (7.6% +/- 0.3% vs. 4.9% +/- 0.2%, respectively, p < 0.001). Significantly higher frequency of MN was observed in both HD and PD patients compared to controls (7.7% +/- 0.5% vs. 4.9% +/- 0.2%, p < 0.001 and 7.5% +/- 0.5% vs. 4.9% +/- 0.2%, p < 0.001, respectively). Multivariate analysis was performed, and it showed that the low-density lipoprotein level was the only independent determinant of increasing MN frequency in our patients (beta = 0.16, t = 2.172, p < 0.05). There is no significant difference in terms of genomic damage between two dialysis modalities, which suggests that PD may not be a more reliable choice in terms of genomic damage.