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dc.contributor.authorÇAYCI, YELİZ TANRIVERDİ
dc.contributor.authorUzun, Meltem
dc.contributor.authorAkbal, Ahmet Ugur
dc.contributor.authorBİRİNCİ, ASUMAN
dc.contributor.authorDURUPINAR, BELMA
dc.contributor.authorÇOBAN, AHMET YILMAZ
dc.date.accessioned2021-03-05T17:58:20Z
dc.date.available2021-03-05T17:58:20Z
dc.date.issued2016
dc.identifier.citationÇOBAN A. Y. , Akbal A. U. , Uzun M., ÇAYCI Y. T. , BİRİNCİ A., DURUPINAR B., "Evaluation of crystal violet decolorization assay for minimal inhibitory concentration detection of primary antituberculosis drugs against Mycobacterium tuberculosis isolates", MEMORIAS DO INSTITUTO OSWALDO CRUZ, cilt.111, ss.454-459, 2016
dc.identifier.issn0074-0276
dc.identifier.othervv_1032021
dc.identifier.otherav_c8166841-1c1b-464a-8f58-859e6cb54a57
dc.identifier.urihttp://hdl.handle.net/20.500.12627/132606
dc.identifier.urihttps://doi.org/10.1590/0074-02760160082
dc.description.abstractIn this study we evaluated the crystal violet decolorization assay (CVDA) for detection of minimum inhibitory concentration (MIC) of antituberculosis drugs. 53 isolates were tested in this study and 13 of them were multidrug resistant (MDR) isolates. The antibiotics concentrations were 2-0.06 mg/L for isoniazid (INH) and rifampicin (RIF) and were 16-0.25 mg/L for streptomycin (STM) and ethambutol (EMB). Crystal violet (CV-25 mg/L) was added into the microwells on the seventh day of incubation and incubation was continued until decolorization. Decolorization of CV was the predictor of bacterial growth. Overall agreements for four drugs were detected as 98.1%, and the average time was detected as 9.5 +/- 0.89 day after inoculation. One isolate for INH and two isolates for STM were determined resistant in the reference method, but susceptible by the CVDA. One isolate was susceptible to EMB by the reference method, but resistant by the CVDA. All results were concordant for RIF. This study shows that CVDA is a rapid, reliable and suitable for determination of MIC values of Mycobacterium tuberculosis. And it can be used easily especially in countries with limited-sources.
dc.language.isoeng
dc.subjectTemel Tıp Bilimleri
dc.subjectBiyokimya
dc.subjectParazitoloji
dc.subjectYaşam Bilimleri
dc.subjectTemel Bilimler
dc.subjectKlinik Tıp (MED)
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectKlinik Tıp
dc.subjectTROPİKAL TIP
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectBiyoloji ve Biyokimya
dc.subjectPARAZİTOLOJİ
dc.titleEvaluation of crystal violet decolorization assay for minimal inhibitory concentration detection of primary antituberculosis drugs against Mycobacterium tuberculosis isolates
dc.typeMakale
dc.relation.journalMEMORIAS DO INSTITUTO OSWALDO CRUZ
dc.contributor.departmentOndokuz Mayıs Üniversitesi , ,
dc.identifier.volume111
dc.identifier.issue7
dc.identifier.startpage454
dc.identifier.endpage459
dc.contributor.firstauthorID233627


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