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dc.contributor.authorPersche, Frank H.
dc.contributor.authorFitzner, Rudolf G.
dc.contributor.authorGonzalez, Jose-B
dc.contributor.authorFimmel, Sabine
dc.contributor.authorKalayciyan, Aylin
dc.contributor.authorOrawa, Helmut
dc.contributor.authorZouboulis, Christos C.
dc.contributor.authorOrfanos, Constantin E.
dc.date.accessioned2021-03-05T18:12:35Z
dc.date.available2021-03-05T18:12:35Z
dc.date.issued2007
dc.identifier.citationKalayciyan A., Orawa H., Fimmel S., Persche F. H. , Gonzalez J., Fitzner R. G. , Orfanos C. E. , Zouboulis C. C. , "Nicotine and biochanin A, but not cigarette smoke, induce anti-inflammatory effects on keratinocytes and endothelial cells in patients with Behcet's disease", JOURNAL OF INVESTIGATIVE DERMATOLOGY, cilt.127, ss.81-89, 2007
dc.identifier.issn0022-202X
dc.identifier.otherav_c93878b6-9ff1-4ac4-835e-4949069eb83c
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/133335
dc.identifier.urihttps://doi.org/10.1038/sj.jid.5700492
dc.description.abstractDuring periods of smoking, patients with Behcet's disease have less oral aphthae than in abstinence. To elucidate this observation, human keratinocytes and dermal microvascular endothelial cells (HMEC-1) were incubated with serum of 20 patients with Behcet's disease and 20 healthy controls for 4 hours. Maximum non-toxic concentrations were determined and the cells were further treated with 6 mu M nicotine, 3.3% cigarette smoke extract (CES), 100 mu M biochanin A, and 6.25/12.5 mu M pyrrolidine dithiocarbamate alone and in combinations for 24 hours. Serum IL-8 levels of patients were significantly lower than those of controls. However, after 4 hours incubation with patients' sera, IL-8 release by both cell types was markedly increased when compared with the corresponding serum levels. The levels of IL-6 and vascular endothelial growth factor (VEGF) release were after 4 hours similar with the corresponding levels in serum. IL-1 was not detected. Nicotine significantly decreased IL-8 and -6 release by HMEC-1 maintained in both patients' and controls' sera, but only IL-6 release by keratinocytes maintained in patients' sera. VEGF release by both cells was markedly increased after nicotine treatment in either serum. CES significantly decreased IL-8 release and increased production of VEGF in keratinocytes maintained in patients' serum. The phytoestrogen biochanin A alone and in combination with nicotine further decreased the secretion of IL-8, -6, and VEGF in all experimental settings. Our data support a specific anti-inflammatory effect of nicotine on keratinocytes and endothelial cells maintained in the serum of patients with Behcet's disease. Moreover, biochanin A is likely to exhibit similar and even more profound results than nicotine.
dc.language.isoeng
dc.subjectSağlık Bilimleri
dc.subjectDERMATOLOJİ
dc.subjectKlinik Tıp
dc.subjectKlinik Tıp (MED)
dc.subjectTıp
dc.subjectDermatoloji
dc.subjectDahili Tıp Bilimleri
dc.titleNicotine and biochanin A, but not cigarette smoke, induce anti-inflammatory effects on keratinocytes and endothelial cells in patients with Behcet's disease
dc.typeMakale
dc.relation.journalJOURNAL OF INVESTIGATIVE DERMATOLOGY
dc.contributor.department, ,
dc.identifier.volume127
dc.identifier.issue1
dc.identifier.startpage81
dc.identifier.endpage89
dc.contributor.firstauthorID181457


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