dc.contributor.author | Ozturk, S | |
dc.contributor.author | Bermek, E | |
dc.contributor.author | Cirakoglu, B | |
dc.date.accessioned | 2021-03-05T18:26:26Z | |
dc.date.available | 2021-03-05T18:26:26Z | |
dc.date.issued | 2003 | |
dc.identifier.citation | Ozturk S., Cirakoglu B., Bermek E., "Partial characterization of the human serum transferrin epitope reactive with the monoclonal antibody TRC-2", HYBRIDOMA AND HYBRIDOMICS, cilt.22, ss.165-171, 2003 | |
dc.identifier.issn | 0272-457X | |
dc.identifier.other | av_ca59f643-77b7-4960-adea-62b7b3f45f8c | |
dc.identifier.other | vv_1032021 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/134043 | |
dc.identifier.uri | https://doi.org/10.1089/153685903322286584 | |
dc.description.abstract | A murine monoclonal antibody (MAb) (TRC-2) specific for human serum transferrin (Tf-h) was developed. This antibody was depressive on cell growth in serum-free medium in the presence of limiting amounts of Tfh, but it did not inhibit the binding of Tf-h-alkaline phosphatase (AP) conjugate to the Tf-receptor (TfR) in a cellular enzyme-linked immunosorbent assay (CELISA) system. On the other hand, the immune complex Tf-h-TRC-2 was implicated to bind to the receptor in indirect CELISA. Moreover, the detectability of Tf-h-TfR on the cell surface via Tf-bound TRC-2 suggested that the antibody may inhibit the rapid internalization of this complex. To map the TRC-2-specific epitope, Tf-h was subjected to proteolytic degradation following sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The treatment with trypsin gave rise to, among others, a fragment of about 42 kDa, which was reactive with TRC-2. Through sequence analysis by automated Edman degradation, the N-terminal sequence of the 42 kDa-tryptic fragment was aligned to the N-terminus of mature transferrin (VPDKTVR). The N-terminal sequence of an immunoreactive CNBr-fragment of about 13 kDa was, in turn, identical with the sequence (NQLRGKK) corresponding to the residues 110-116 on Tf-h. | |
dc.language.iso | eng | |
dc.subject | Mikrobiyoloji | |
dc.subject | İmmünoloji | |
dc.subject | BİYOKİMYA VE MOLEKÜLER BİYOLOJİ | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Tıp | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Temel Tıp Bilimleri | |
dc.subject | Biyokimya | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Biyoteknoloji | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Sitogenetik | |
dc.subject | Temel Bilimler | |
dc.subject | BİYOKİMYASAL ARAŞTIRMA YÖNTEMLERİ | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Biyoloji ve Biyokimya | |
dc.subject | BİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ | |
dc.title | Partial characterization of the human serum transferrin epitope reactive with the monoclonal antibody TRC-2 | |
dc.type | Makale | |
dc.relation.journal | HYBRIDOMA AND HYBRIDOMICS | |
dc.contributor.department | , , | |
dc.identifier.volume | 22 | |
dc.identifier.issue | 3 | |
dc.identifier.startpage | 165 | |
dc.identifier.endpage | 171 | |
dc.contributor.firstauthorID | 168751 | |