Leptin Gene Polymorphisms in Native Turkish Cattle Breeds

Abstract

The aim of the study was to determine leptin gene polymorphisms in South Anatolian Red (SAR), East Anatolian Red (EAR) and Turkish Grey Cattle. In the study unrelated 40 SAR. 40 EAR and 40 Turkish Grey cattle were used. Target sites in leptin gene exon 2, exon 3 and intron 2 were amplified by polymerase chain reaction (PCR). The single nucleotide polymorphism (SNP) consisting site in exon 2, exon 3 and intron 2 were determined as a result of digestion with Kpn2l. HphI and Sau3Al restriction enzymes, respectively. The highest T allele frequency related with production traits for Kpn2l polymorphism was found for SAR cattle. For HphI polymorphism. T allele frequencies were detected clearly predominant. Within each breed for Sau3Al polymorphism B and C allele frequencies that effect production traits were found to be dramatically lower than A allele frequency. As a result we can suggest that there was no clearly difference that can create any advantage in terms of leptin gene SNPs among the three native Turkish cattle breeds.