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dc.contributor.authorBaki, Sefa
dc.contributor.authorGuclu, Kubilay
dc.contributor.authorOzyurek, Mustafa
dc.contributor.authorApak, Resat
dc.contributor.authorTufan, Ayse Nur
dc.date.accessioned2021-03-06T07:23:47Z
dc.date.available2021-03-06T07:23:47Z
dc.date.issued2014
dc.identifier.citationTufan A. N. , Baki S., Guclu K., Ozyurek M., Apak R., "A Novel Differential Pulse Voltammetric (DPV) Method for Measuring the Antioxidant Capacity of Polyphenols-Reducing Cupric Neocuproine Complex", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, cilt.62, ss.7111-7117, 2014
dc.identifier.issn0021-8561
dc.identifier.otherav_dc65c4eb-1644-4bc3-9f71-4df988f9bb74
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/145253
dc.identifier.urihttps://doi.org/10.1021/jf5017797
dc.description.abstractA novel differential pulse voltammetric (DPV) method is presented, using a chromogenic oxidizing reagent, cupric neocuproine complex (Cu(Nc)(2)(2+)), for the assessment of antioxidant capacity of polyphenolic compounds (i.e., flavonoids, simple phenolic acids, and hydroxycinnamic acids), ascorbic acid, and real samples for the first time. The electrochemical behavior of the Cu(Nc)(2)(2+) complex was studied by cyclic voltammetry at a glassy carbon (GC) electrode. The electroanalytical method was based on the reduction of Cu(Nc)(2)(2+) to Cu(Nc)(2)(+) by antioxidants and electrochemical detection of the remaining Cu(II)-Nc (unreacted complex), the difference being correlated to antioxidant capacity of the analytes. The calibration curves of individual compounds comprising polyphenolics and vitamin C were constructed, and their response sensitivities and linear concentration ranges were determined. The reagent on the GC electrode retained its reactivity toward antioxidants, and the measured trolox equivalent antioxidant capacity (TEAC) values of various antioxidants suggested that the reactivity of the Cu(II)-Nc reagent is comparable to that of the solution-based spectrophotometric cupric ion reducing antioxidant capacity (CUPRAC) assay. This electroanalytical method better tolerated sample turbidity and provided higher sensitivity (i.e., lower detection limits) in antioxidant determination than the spectrophotometric assay. The proposed method was successfully applied to the measurement of total antioxidant capacity (TAC) in some herbal tea samples such as green tea, sage, marjoram, and alchemilla. Results demonstrated that the proposed voltammetric method has precision and accuracy comparable to those of the spectrophotometric CUPRAC assay.
dc.language.isoeng
dc.subjectZiraat
dc.subjectBitki Koruma
dc.subjectFitopatoloji
dc.subjectBiyoteknoloji ve Genetik
dc.subjectGıda Mühendisliği
dc.subjectDiğer
dc.subjectTemel Bilimler
dc.subjectMühendislik ve Teknoloji
dc.subjectGIDA BİLİMİ VE TEKNOLOJİSİ
dc.subjectTarımsal Bilimler
dc.subjectTemel Bilimler (SCI)
dc.subjectKimya
dc.subjectKİMYA, UYGULAMALI
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectTarım Bilimleri
dc.subjectTARIM, MULTİDİSİPLİNLER
dc.titleA Novel Differential Pulse Voltammetric (DPV) Method for Measuring the Antioxidant Capacity of Polyphenols-Reducing Cupric Neocuproine Complex
dc.typeMakale
dc.relation.journalJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
dc.contributor.departmentİstanbul Üniversitesi , ,
dc.identifier.volume62
dc.identifier.issue29
dc.identifier.startpage7111
dc.identifier.endpage7117
dc.contributor.firstauthorID11372


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