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dc.contributor.authorKaraismailoglu, Eda
dc.contributor.authorKORKUSUZ, PETEK
dc.contributor.authorAydin, Makbule Cisel
dc.contributor.authorAKAR, İREM
dc.contributor.authorUSUBÜTÜN, ALP
dc.contributor.authorBİLGİÇ, ELİF
dc.contributor.authorMeydanli, Elif Guzel
dc.contributor.authorKose, Sevil
dc.date.accessioned2021-03-02T23:01:20Z
dc.date.available2021-03-02T23:01:20Z
dc.date.issued2017
dc.identifier.citationBİLGİÇ E., Meydanli E. G. , Kose S., Aydin M. C. , Karaismailoglu E., AKAR İ., USUBÜTÜN A., KORKUSUZ P., "Endocannabinoids modulate apoptosis in endometriosis and adenomyosis", ACTA HISTOCHEMICA, cilt.119, sa.5, ss.523-532, 2017
dc.identifier.issn0065-1281
dc.identifier.otherav_0ffa15ab-80ac-4aa7-a986-492800e0fb08
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/16266
dc.identifier.urihttps://doi.org/10.1016/j.acthis.2017.05.005
dc.description.abstractAdenomyosis that is a form of endometriosis is the growth of ectopic endometrial tissue within the muscular wall of the uterus (myometrium), which may cause dysmenorrhea and infertility. Endocannabinoid mediated apoptotic mechanisms of endometriosis and adenomyosis are not known. We hypothesized that the down regulation of endocannabinoid receptors and/or alteration in their regulatory enzymes may have a direct role in the pathogenesis of endometriosis and adenomyosis through apoptosis. Endocannabinoid receptors CB1 and CB2, their synthesizing and catabolizing enzymes (FAAH, NAPE-PLD, DAGL, MAGL) and the apoptotic indexes were immunohistochemically assessed in endometriotic and adenomyotic tissues. Findings were compared to normal endometrium and myometrium. Endometrial adenocarcinoma (Ishikawa) and ovarian endometriosis cyst wall stromal (CRL-7566) cell lines were furthermore cultured with or without cannabinoid receptor agonists. The IC50 value for CB1 and CB2 receptor agonists was quantified. Cannabinoid agonists on cell death were investigated by Annexin-V/Propidium iodide labeling with flow cytometry. CB1 and CB2 receptor levels decreased in endometriotic and adenomyotic tissues compared to the control group (p = 0,001 and p = 0,001). FAAH, NAPE-PLD, MAGL and DAGL enzyme levels decreased in endometriotic and adenomyotic tissues compared to control (p = 0,001, p = 0,001, p = 0,001 and p = 0,002 respectively). Apoptotic cell indexes both in endometriotic and adenomyotic tissues also decreased significantly, compared to the control group (p = 0,001 and p = 0,001). CB1 and CB2 receptor agonist mediated dose dependent fast anti-proliferative and pro-apoptotic effects were detected in Ishikawa and ovarian endometriosis cyst wall stromal cell lines (CRI, 7566). Endocannabinoids are suggested to increase apoptosis mechanisms in endometriosis and adenomyosis. CB1 and CB2 antagonists can be considered as potential medical therapeutic agents for endometriosis and adenomyosis.
dc.language.isoeng
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectTemel Bilimler
dc.subjectTemel Tıp Bilimleri
dc.subjectYaşam Bilimleri
dc.subjectHistoloji-Embriyoloji
dc.subjectSağlık Bilimleri
dc.subjectTıp
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectHÜCRE BİYOLOJİSİ
dc.titleEndocannabinoids modulate apoptosis in endometriosis and adenomyosis
dc.typeMakale
dc.relation.journalACTA HISTOCHEMICA
dc.contributor.departmentHacettepe Üniversitesi , Tıp Fakültesi (Türkçe) , Histoloji Ve Embriyoloji A.B.D. (Türkçe)
dc.identifier.volume119
dc.identifier.issue5
dc.identifier.startpage523
dc.identifier.endpage532
dc.contributor.firstauthorID238849


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