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dc.contributor.authorEdis, Bilge
dc.contributor.authorUnlu, Ayhan
dc.contributor.authorHaciosmanoglu, Ebru
dc.contributor.authorVarol, Basak
dc.contributor.authorBektas, Muhammet
dc.date.accessioned2021-03-06T19:57:05Z
dc.date.available2021-03-06T19:57:05Z
dc.date.issued2017
dc.identifier.citationEdis B., Varol B., Haciosmanoglu E., Unlu A., Bektas M., "Cross-reacting material 197 (CRM197) affects actin cytoskeleton of endothelial cells", GENERAL PHYSIOLOGY AND BIOPHYSICS, cilt.36, ss.383-389, 2017
dc.identifier.issn0231-5882
dc.identifier.othervv_1032021
dc.identifier.otherav_f8827701-a297-4623-84bb-c3cd5672fc0a
dc.identifier.urihttp://hdl.handle.net/20.500.12627/162778
dc.identifier.urihttps://doi.org/10.4149/gpb_2017006
dc.description.abstractCRM197, cross-reacting material 197, is a mutant of diphtheria toxin (DTx). CRM197 is used in pharmacology as a carrier protein. It has been recently shown that CRM197 causes breakdown in actin filaments. In order to show intracellular localization of CRM197 and visualize cell structure via actin cytoskeleton, endothelial cells were cultured and subjected to CRM197 in vitro. To address the interaction between CRM197 and actin both experimental and theoretical studies were carried out. Colocalization of CRM197 with actin filaments was determined by immunofluorescence microscopy. Following 24-hour incubation, the loss of cell-cell contact between cells was prominent. CRM197 was shown to bind to G-actin by gel filtration chromatography, and this binding was confirmed by Western blot analysis of eluted samples obtained following chromatography. Based on crystal structure, docked model of CRM197-actin complex was generated. Molecular dynamics simulation revealed that Lys42, Cys218, Cys233 of CRM197 interacts with Gly197, Arg62 and Ser60 of G-actin, respectively. CRM197 binding to G-actin, colocalization of CRM197 with actin filament, and actin cytoskeleton rearrangement resulting in the loss of cell-cell contact show that actin comes into sight as target molecule for CRM197.
dc.language.isoeng
dc.subjectClinical Biochemistry
dc.subjectCancer Research
dc.subjectMolecular Biology
dc.subjectDrug Discovery
dc.subjectAging
dc.subjectPhysiology
dc.subjectGeneral Biochemistry, Genetics and Molecular Biology
dc.subjectBiochemistry
dc.subjectBiophysics
dc.subjectPhysiology (medical)
dc.subjectBiochemistry (medical)
dc.subjectLife Sciences
dc.subjectHealth Sciences
dc.subjectStructural Biology
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectBİYOFİZİK
dc.subjectBiyoloji ve Biyokimya
dc.subjectFİZYOLOJİ
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectBiyofizik
dc.subjectBiyokimya
dc.subjectFizyoloji
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectSitogenetik
dc.subjectTemel Bilimler
dc.subjectBiochemistry, Genetics and Molecular Biology (miscellaneous)
dc.titleCross-reacting material 197 (CRM197) affects actin cytoskeleton of endothelial cells
dc.typeMakale
dc.relation.journalGENERAL PHYSIOLOGY AND BIOPHYSICS
dc.contributor.departmentİstanbul Üniversitesi , ,
dc.identifier.volume36
dc.identifier.issue4
dc.identifier.startpage383
dc.identifier.endpage389
dc.contributor.firstauthorID246344


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