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dc.contributor.authorErgun, Yağmur
dc.contributor.authorGünel, Tuba
dc.contributor.authorSevgin, Kübra
dc.contributor.authorGöksever Çelik, Hale
dc.contributor.authorKarahüseyinoğlu, Serçin
dc.contributor.authorKamalı Şimşek, Nilüfer
dc.contributor.authorBenian, Ali
dc.date.accessioned2021-12-10T10:08:26Z
dc.date.available2021-12-10T10:08:26Z
dc.date.issued2021
dc.identifier.citationKamalı Şimşek N., Benian A., Sevgin K., Ergun Y., Göksever Çelik H., Karahüseyinoğlu S., Günel T., "Microrna analysis of human decidua mesenchymal stromal cells from preeclampsia patients", Placenta, sa.115, ss.12-19, 2021
dc.identifier.issn0143-4004
dc.identifier.othervv_1032021
dc.identifier.otherav_2b252d11-c5d5-4fe9-936a-dd599324b055
dc.identifier.urihttp://hdl.handle.net/20.500.12627/169269
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S014340042100583X
dc.identifier.urihttps://doi.org/10.1016/j.placenta.2021.09.004
dc.description.abstractIntroduction: In preeclampsia (PE), human decidua mesenchymal stromal cells (hDMSCs) are exposed toabnormally high levels of oxidative stress and inflammatory factors circulating in the maternal blood. MicroRNAs(miRNAs) have been shown to have a significant impact on the differentiation, maturation and function ofmesenchymal stromal cells (MSCs). Our aim in the present study is firstly to investigate differentially expressedmiRNA levels to be used as a biomarker in the early detection of PE and secondly to investigate whether thosedifferentially expressed miRNAs in hDMSCs have an effect on the pathogenesis of PE.Methods: This study covers miRNA expression analysis of hDMSCs from 7 PE patient and 7 healthy pregnantwomen and is a preliminary study to investigate putative biomarkers. After cell culture and cell sorting, totalRNA including miRNAs were isolated from hDMSCs. Let-7b-3p, let-7f-1-3p, miR-191–3p, miR-550a-5p, miR-33b-3p and miR-425–3p were used for miRNA analysis and U6 snRNA was used for normalization of the samples.MiRNA analysis was performed by droplet digital polymerase chain reaction (ddPCR) method and obtainedresults were evaluated statistically.Results: As a result of the analysis, it was observed that the levels of hsa-miR-33b-3p significantly (AUC: 0.93, p =0.04, fold change: 4.5) increased in hDMSC of PE patients compared to healthy controls. However, let-7b-3p, let-7f-1-3p, miR-191–3p, miR-550a-5p, and miR-425–3p were not considered as significant because they did notmeet the p < 0,05 requirement.Discussion: Within the scope of the study, it is predicted that miR-33b-3p (p = 0.004, AUC = 0.93) can be used asa biomarker in detecting PE.
dc.language.isoeng
dc.subjectMultidisciplinary
dc.subjectDoğa Bilimleri Genel
dc.subjectÇOK DİSİPLİNLİ BİLİMLER
dc.subjectTemel Bilimler
dc.subjectTemel Bilimler (SCI)
dc.titleMicrorna analysis of human decidua mesenchymal stromal cells from preeclampsia patients
dc.typeMakale
dc.relation.journalPlacenta
dc.contributor.departmentBahçeşehir Üniversitesi , Fen-Edebiyat Fakültesi , Genetik Ve Biyoinformatik Bölümü
dc.identifier.issue115
dc.identifier.startpage12
dc.identifier.endpage19
dc.contributor.firstauthorID2724700


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