Antibacterial effects of saliva substitutes containing lysozyme or lactoferrin against Streptococcus mutans.

Abstract

Objective: To determine the antibacterial effects of different saliva-substitutes-containing-lysozyme(LYZ) or-lac-toferrin(LF) on Streptococcus mutans(S. mutans) in comparison with human saliva. Design: In vitro wound-healing assay was performed with L929 mouse fibroblast cell line by using various con-centrations of LYZ and LF to determine optimum concentrations and to confirm do not show any cytotoxicity of proteins according to cell culture studies. Antibacterial effect was assessed by determining Minimum Inhibitory Concentrations for all groups on S.mutans. Bacterial adhesion of S. mutans for 4 h on hydroxyapatite(HAP) discs after application of different saliva substitutes was evaluated. The formulations were:saliva-substitute(Group SS); saliva-substitute-containing-Lactoferrin(Group SSLF);saliva-substitute-containing-Lysozyme(Group SSLYZ). Human saliva was control group(Group HS). Results: In vitro wound healing assay results showed that, when added into the cell culture media, LYZ and LF significantly increase 48-h scratch wound closure compared to the cell culture media(p 0.05). Conclusions: The study of cell viability and wound healing was great significance in the optimum concentrations of LYZ and LF. Among formulations, saliva-substitutes-containing-LYZ or-LF exhibited higher inhibitory effect on S.mutans.