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dc.contributor.authorCifteci, Asuman
dc.contributor.authorAPAK, Mustafa Reşat
dc.contributor.authorÇELİK, Saliha Esin
dc.date.accessioned2021-12-10T10:49:58Z
dc.date.available2021-12-10T10:49:58Z
dc.identifier.citationCifteci A., ÇELİK S. E. , APAK M. R. , "Gold-Nanoparticle Based Turn-on Fluorometric Sensor for Quantification of Sulfhydryl and Disulfide Forms of Biothiols: Measurement of Thiol/Disulfide Homeostasis", ANALYTICAL LETTERS, 2021
dc.identifier.issn0003-2719
dc.identifier.othervv_1032021
dc.identifier.otherav_572b98e0-2c3f-4df7-b82a-5d4d2936e49f
dc.identifier.urihttp://hdl.handle.net/20.500.12627/170673
dc.identifier.urihttps://doi.org/10.1080/00032719.2021.1958830
dc.description.abstractA simple and sensitive method was developed to determine biothiols in both sulfhydryl (-SH) and disulfide (S-S) forms using a fluorometric sensor composed of citrate-stabilized gold nanoparticles (AuNPs) prepared by electrostatic adsorption of water-soluble fluorophore Rhodamine-6G (Rh-6G) dye onto citrate-stabilized AuNPs resulting in the fluorescence resonance energy transfer in the turn-off mode. Due to the high surface affinities of biothiols to AuNPs, the fluorescence increment at 552 nm (turn-on mode) accompanying the release of Rh-6G indicated the thiol content. This assay also enables the differentiation of oxidized and reduced thiols and the measurement of thiol/disulfide homeostasis as a vital oxidative stress indicator using thiol-alkylating agent N-ethylmaleimide (NEM). After pretreatment with NEM, disulfides were measured in the presence of reduced thiols. The overall biothiol levels and thiol/disulfide ratios were measured using the proposed assay. This method was shown to be selective over various potential interfering compounds (e.g., polyphenols and plasma antioxidants) known to adversely affect redox-based assays. The proposed assay also enabled the naked-eye detection of biothiols by NP agglomeration and may be successfully applied to pharmaceutical samples containing thiols.
dc.language.isoeng
dc.subjectChemistry (miscellaneous)
dc.subjectGeneral Chemistry
dc.subjectPhysical Sciences
dc.subjectAnalytical Chemistry
dc.subjectFiltration and Separation
dc.subjectTemel Bilimler
dc.subjectAnalitik Kimya
dc.subjectTemel Bilimler (SCI)
dc.subjectKİMYA, ANALİTİK
dc.subjectKimya
dc.titleGold-Nanoparticle Based Turn-on Fluorometric Sensor for Quantification of Sulfhydryl and Disulfide Forms of Biothiols: Measurement of Thiol/Disulfide Homeostasis
dc.typeMakale
dc.relation.journalANALYTICAL LETTERS
dc.contributor.departmentİstanbul Üniversitesi-Cerrahpaşa , ,
dc.contributor.firstauthorID2703163


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