Studies on diagnosis of spontaneous Gumboro disease (IBD) by histopathological and immunoperoxidase methods

Abstract

In this study, organ and tissue samples were collected for routine histopathological examinations and also for detection and definitive diagnosis of Gumboro disease (IBD) viral antigens by immunoperoxidase methods during postmortem examinations of dead or sacrificed birds taken from various houses which are localized within regions of intensive poultry production in Turkey (Istanbul, Adapazan, Bolu, Balikesir), where this disease is seen quite frequently and is still a major cause of economical losses in poultry industry in our country. In total, 50 birds were examined for the suspected disease. Sections prepared from collected organs or tissues were processed for both routine histopathological examinations and also for immunohistochemical evaluations under fight microscope by StreptAvidin-Biotin Peroxidase Complex Method.