dc.contributor.author | GÜNGÖR, Zeynep Banu | |
dc.contributor.author | Dicle, Diclehan Ali | |
dc.contributor.author | ÖZYÜREK, Mustafa | |
dc.contributor.author | KUCUR, Mine | |
dc.contributor.author | Kamis, Handan | |
dc.contributor.author | Filik, Hayati | |
dc.contributor.author | AVAN, Asiye Aslıhan | |
dc.contributor.author | ALTAŞ PUNTAR, Nilay | |
dc.date.accessioned | 2021-12-10T13:19:21Z | |
dc.date.available | 2021-12-10T13:19:21Z | |
dc.identifier.citation | Filik H., AVAN A. A. , ALTAŞ PUNTAR N., ÖZYÜREK M., GÜNGÖR Z. B. , KUCUR M., Kamis H., Dicle D. A. , "Ethylenediamine grafted carbon nanotube aerogels modified screen-printed electrode for simultaneous electrochemical immunoassay of multiple tumor markers", JOURNAL OF ELECTROANALYTICAL CHEMISTRY, cilt.900, 2021 | |
dc.identifier.issn | 1572-6657 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_fed0847c-d2ae-4606-b9a6-9efeafe0d3c8 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/175931 | |
dc.identifier.uri | https://doi.org/10.1016/j.jelechem.2021.115700 | |
dc.description.abstract | A sensitive sandwich-model immunoassay designed for simultaneous electrochemical measurement of alphafetoprotein and carcinoembryonic antigen handling multiple-label strategy. The sandwich-model immunosensor was constructed by assembling ethylenediamine-MWCNT aerogels (EDA-CAGs), AuNPs and capture antibodies (Cp-Ab1) on the screen-printed carbon electrode (SPCE). The prepared EDA-CAG textures were analyzed using Fourier transform infrared (FT-IR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray Spectroscopy (EDX). In this protocol, AuNPs decorated EDA-CAG-carried Thionine (Thi) and AuNPs decorated EDA-CAG-carried Safranine O (SfO) (denoted as AuNP-Thi-EDA-CAG and AuNP-SfOEDA-CAG, respectively) were properly exploited as distinguishable signal labels, which were used to attach detection antibodies (Ab2) (anti-AFP2 and anti-CEA2), respectively. When two tumor antigens were present, a square wave voltammetry (SWV) scan displayed two well-separated signals, each signal indicated one target antigen. Observed results introduced that the square wave voltammetric peak current exhibited a good linear relationship to logarithm concentration in the ranges from 0.005 to 1.0 ng/mL for both analytes. The LoDs were 0.0015 and 0.0010 ng/mL (at signal/noise S/N = 3) for AFP and CEA, respectively. The designed sandwich-type immunosensor was applied to real serum sample analysis. | |
dc.language.iso | eng | |
dc.subject | Temel Bilimler | |
dc.subject | Analytical Chemistry | |
dc.subject | Filtration and Separation | |
dc.subject | Electrochemistry | |
dc.subject | Chemistry (miscellaneous) | |
dc.subject | General Chemistry | |
dc.subject | Physical Sciences | |
dc.subject | Fizikokimya | |
dc.subject | Elektrokimya | |
dc.subject | Analitik Kimya | |
dc.subject | ELEKTROKİMYA | |
dc.subject | Temel Bilimler (SCI) | |
dc.subject | Kimya | |
dc.subject | KİMYA, ANALİTİK | |
dc.title | Ethylenediamine grafted carbon nanotube aerogels modified screen-printed electrode for simultaneous electrochemical immunoassay of multiple tumor markers | |
dc.type | Makale | |
dc.relation.journal | JOURNAL OF ELECTROANALYTICAL CHEMISTRY | |
dc.contributor.department | İstanbul Üniversitesi , , | |
dc.identifier.volume | 900 | |
dc.contributor.firstauthorID | 2750458 | |