dc.contributor.author | Millo, Diego | |
dc.contributor.author | Hildebrandt, Peter | |
dc.contributor.author | SEZER, Murat | |
dc.contributor.author | Zebger, Ingo | |
dc.contributor.author | Weidinger, Inez M. | |
dc.date.accessioned | 2022-02-18T09:13:34Z | |
dc.date.available | 2022-02-18T09:13:34Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | SEZER M., Millo D., Weidinger I. M. , Zebger I., Hildebrandt P., "Analyzing the catalytic processes of immobilized redox enzymes by vibrational spectroscopies", IUBMB LIFE, cilt.64, sa.6, ss.455-464, 2012 | |
dc.identifier.issn | 1521-6543 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_266e9cce-320a-4f3d-8f72-f80512c3e6ff | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/176784 | |
dc.identifier.uri | https://doi.org/10.1002/iub.1020 | |
dc.description.abstract | Analyzing the structure and function of redox enzymes attached to electrodes is a central challenge in many fields of fundamental and applied life science. Electrochemical techniques such as cyclic voltammetry which are routinely used do not provide insight into the molecular structure and reaction mechanisms of the immobilized proteins. Surface-enhanced infrared absorption (SEIRA) and surface-enhanced resonance Raman (SERR) spectroscopy may fill this gap, if nanostructured Au or Ag are used as conductive support materials. In this account, we will first outline the principles of the methodology including a description of the most important strategies for biocompatible protein immobilization. Subsequently, we will critically review SERR and SEIRA spectroscopic approaches to characterize the protein and active site structure of the immobilized enzymes. Special emphasis is laid on the combination of surface-enhanced vibrational spectroscopies with electrochemical methods to analyze equilibria and dynamics of the interfacial redox processes. Finally, we will assess the potential of SERR and SEIRA spectroscopy for in situ investigations on the basis of the first promising studies on human sulfite oxidase and hydrogenases under turnover conditions. (C) 2012 IUBMB IUBMB Life, 2012 | |
dc.language.iso | eng | |
dc.subject | Aging | |
dc.subject | BİYOKİMYA VE MOLEKÜLER BİYOLOJİ | |
dc.subject | General Biochemistry, Genetics and Molecular Biology | |
dc.subject | Biochemistry | |
dc.subject | Structural Biology | |
dc.subject | Life Sciences | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Sitogenetik | |
dc.subject | Temel Bilimler | |
dc.subject | Biochemistry, Genetics and Molecular Biology (miscellaneous) | |
dc.subject | Clinical Biochemistry | |
dc.subject | Cell Biology | |
dc.subject | Cancer Research | |
dc.subject | Molecular Biology | |
dc.subject | Drug Discovery | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | HÜCRE BİYOLOJİSİ | |
dc.subject | Tıp | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Temel Tıp Bilimleri | |
dc.subject | Histoloji-Embriyoloji | |
dc.subject | Yaşam Bilimleri | |
dc.title | Analyzing the catalytic processes of immobilized redox enzymes by vibrational spectroscopies | |
dc.type | Makale | |
dc.relation.journal | IUBMB LIFE | |
dc.contributor.department | Technical University of Berlin , , | |
dc.identifier.volume | 64 | |
dc.identifier.issue | 6 | |
dc.identifier.startpage | 455 | |
dc.identifier.endpage | 464 | |
dc.contributor.firstauthorID | 3379946 | |