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dc.contributor.authorATMACA, HARİKA
dc.contributor.authorBilir, Ayhan
dc.contributor.authorUYSAL, AYŞEGÜL
dc.contributor.authorSercan, Ogun
dc.contributor.authorAyla, Sule
dc.contributor.authorÖKTEM, GÜLPERİ
dc.contributor.authorUslu, Ruchan
dc.contributor.authorInan, Sevinc V.
dc.contributor.authorDemiray, Sirin B.
dc.date.accessioned2021-03-02T23:25:25Z
dc.date.available2021-03-02T23:25:25Z
dc.date.issued2014
dc.identifier.citationÖKTEM G., Bilir A., Uslu R., Inan S. V. , Demiray S. B. , ATMACA H., Ayla S., Sercan O., UYSAL A., "Expression profiling of stem cell signaling alters with spheroid formation in CD133(high)/CD44(high) prostate cancer stem cells", ONCOLOGY LETTERS, cilt.7, sa.6, ss.2103-2109, 2014
dc.identifier.issn1792-1074
dc.identifier.othervv_1032021
dc.identifier.otherav_12579ec2-46d0-4567-9562-0d87c6e03e8e
dc.identifier.urihttp://hdl.handle.net/20.500.12627/17787
dc.identifier.urihttps://doi.org/10.3892/ol.2014.1992
dc.description.abstractCancer stem cells (CSC) isolated from multiple tumor types differentiate in vivo and in vitro when cultured in serum; however, the factors responsible for their differentiation have not yet been identified. The first aim of the present study was to identify CD133(high)/CD44(high) DU145 prostate CSCs and compare their profiles with non-CSCs as bulk counterparts of the population. Subsequently, the two populations continued to be three-dimensional multicellular spheroids. Differentiation was then investigated with stem cell-related genomic characteristics. Polymerase chain reaction array analyses of cell cycle regulation, embryonic and mesenchymal cell lineage-related markers, and telomerase reverse transcriptase (TERT) and Notch signaling were performed. Immunohistochemistry of CD117, Notch1, Jagged1, Delta1, Sox2, c-Myc, Oct4, KLF4, CD90 and SSEA1 were determined in CSC and non-CSC monolayer and spheroid subcultures. Significant gene alterations were observed in the CD133(high)/CD44(high) population when cultured as a monolayer and continued as spheroid. In this group, marked gene upregulation was determined in collagen type 9 a1, Islet1 and cyclin D2. Jagged1, Delta-like 3 and Notch1 were respectively upregulated genes in the Notch signaling pathway. According to immunoreactivity, the staining density of Jagged1, Sox2, Oct4 and Klf-4 increased significantly in CSC spheroids. Isolated CSCs alter their cellular characterization over the course of time and exhibit a differentiation profile while maintaining their former surface antigens at a level of transcription or translation. The current study suggested that this differentiation process may be a mechanism responsible for the malignant process and tumor growth.
dc.language.isoeng
dc.subjectTıp
dc.subjectONKOLOJİ
dc.subjectKlinik Tıp
dc.subjectKlinik Tıp (MED)
dc.subjectSağlık Bilimleri
dc.subjectDahili Tıp Bilimleri
dc.subjectİç Hastalıkları
dc.subjectOnkoloji
dc.titleExpression profiling of stem cell signaling alters with spheroid formation in CD133(high)/CD44(high) prostate cancer stem cells
dc.typeMakale
dc.relation.journalONCOLOGY LETTERS
dc.contributor.departmentEge Üniversitesi , Tıp Fakültesi , Histoloji Ve Embriyoloji Ana Bilim Dalı
dc.identifier.volume7
dc.identifier.issue6
dc.identifier.startpage2103
dc.identifier.endpage2109
dc.contributor.firstauthorID215254


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