Show simple item record

dc.contributor.authorKalem, Hatice Hande
dc.contributor.authorIrez, Tulay
dc.contributor.authorTulay, Pinar
dc.date.accessioned2022-02-18T10:24:57Z
dc.date.available2022-02-18T10:24:57Z
dc.identifier.citationKalem H. H. , Tulay P., Irez T., "How does sperm apoptosis affect the outcome of intrauterine insemination and intracytoplasmic sperm injection?", ANDROLOGIA, 2022
dc.identifier.issn0303-4569
dc.identifier.othervv_1032021
dc.identifier.otherav_9a6dd32b-30d9-4031-a6d7-3649f6abcece
dc.identifier.urihttp://hdl.handle.net/20.500.12627/179201
dc.identifier.urihttps://doi.org/10.1111/and.14381
dc.description.abstractUp to 20% of male infertility is caused by abnormal DNA organization of the sperm and anomalies of the sperm apoptosis. The aim of this study was to investigate the sperm DNA apoptosis and viability in patients undergoing intrauterine insemination (IUI) and intracytoplasmic sperm injection (ICSI). In the second part of the analysis, sperm DNA apoptosis and viability were investigated in patients with oligozoospermia and normospermia respectively. A total of 45 IUI and 38 ICSI patients were included in this study. Annexin V analysis was performed to investigate the sperm viability, and TUNEL assay was used to evaluate the sperm DNA apoptosis. Further investigations using 12 oligozoospermia and 11 control samples for sperm viability and sperm DNA apoptosis at different incubation periods and temperatures were performed. The results of this study showed a negative correlation between the sperm DNA apoptosis in IUI patients, but no relationship was observed for the ICSI patients. The second part of this study showed that incubation of semen samples at 37 degrees C for 3 h has detrimental effects on the sperm DNA integrity. In conclusion, the incubation of semen at high temperatures affects the sperm quality. The results of this study showed that these tests can be beneficial for the infertile couples to achieve pregnancy.
dc.language.isoeng
dc.subjectİç Hastalıkları
dc.subjectEndokrinoloji ve Metabolizma Hastalıkları
dc.subjectEndocrinology
dc.subjectEndocrine and Autonomic Systems
dc.subjectEndocrinology, Diabetes and Metabolism
dc.subjectReproductive Medicine
dc.subjectLife Sciences
dc.subjectHealth Sciences
dc.subjectSağlık Bilimleri
dc.subjectDahili Tıp Bilimleri
dc.subjectTıp
dc.subjectENDOKRİNOLOJİ VE METABOLİZMA
dc.subjectKlinik Tıp (MED)
dc.subjectKlinik Tıp
dc.subjectANDROLOJİ
dc.titleHow does sperm apoptosis affect the outcome of intrauterine insemination and intracytoplasmic sperm injection?
dc.typeMakale
dc.relation.journalANDROLOGIA
dc.contributor.departmentİstanbul Teknik Üniversitesi , ,
dc.contributor.firstauthorID3390451


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record