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dc.contributor.authorÜstünova, Savaş
dc.contributor.authorYilmazer, Nadim
dc.contributor.authorUvez, Ayca
dc.contributor.authorÖztay, Füsün
dc.contributor.authorArmutak, Elif Ilkay
dc.contributor.authorDimas, Konstantinos
dc.contributor.authorMeral, Ismail
dc.contributor.authorSonmez, Kivilcim
dc.contributor.authorBulut, Huri
dc.contributor.authorEsener, Osman B. Burak
dc.contributor.authorKutucu, Deniz
dc.contributor.authorUtkusavas, Ayfer
dc.contributor.authorGurevin, Ebru Gurel
dc.date.accessioned2022-07-04T12:58:50Z
dc.date.available2022-07-04T12:58:50Z
dc.identifier.citationUtkusavas A., Gurevin E. G. , Yilmazer N., Uvez A., Öztay F., Bulut H., Üstünova S., Esener O. B. B. , Sonmez K., Kutucu D., et al., "Effects of combined administration of doxorubicin and chloroquine on lung pathology in mice with solid Ehrlich ascites carcinoma", BIOTECHNIC & HISTOCHEMISTRY, 2022
dc.identifier.issn1052-0295
dc.identifier.otherav_3481721d-f20b-45c9-af56-26c773f89581
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/182251
dc.identifier.urihttps://doi.org/10.1080/10520295.2022.2036369
dc.description.abstractCombined use of a chemotherapeutic agent and an autophagy inhibitor is a novel cancer treatment strategy. We investigated the effects of chloroquine (CQ) on lung pathology caused by both solid Ehrlich ascites carcinoma (EAC) and doxorubicin (DXR). A control group and eight experimental groups of adult female mice were inoculated subcutaneously with 2.5 x 10(6) EAC cells. DXR (1.5 mg/kg and 3 mg/kg) and CQ (25 mg/kg and 50 mg/kg) alone or in combination were injected intraperitoneally on days 2, 7 and 12 following inoculation with EAC cells. Lung tissue samples were examined using immunohistochemistry (IHC) for endothelial (eNOS), inducible nitric oxide synthase (iNOS) and neutrophil gelatinase-associated lipocalin (NGAL). Serum catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and malondialdehyde (MDA) levels were measured using ELISA. We found decreased levels of iNOS and eNOS in the groups that received 1.5 mg/kg DXR alone and in combination with 25 mg/kg and 50 mg/kg CQ. Combined administration of DXR and CQ partially prevented disruption of alveolar structure. Levels of antioxidant enzymes and MDA were lower in all treated groups; the greatest reduction was observed in mice that received the combination of 25 mg/kg CQ + 1.5 mg/kg DXR. Levels of NGAL were elevated in all treated groups. We found that CQ ameliorated both EAC and DOX induced lung pathology in female mice with solid EAC by reducing oxidative stress.
dc.language.isoeng
dc.subjectMolecular Medicine
dc.subjectTıp
dc.subjectLife Sciences
dc.subjectBİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ
dc.subjectMikrobiyoloji
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectHÜCRE BİYOLOJİSİ
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectHistoloji-Embriyoloji
dc.subjectYaşam Bilimleri
dc.subjectBiyoteknoloji
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectTemel Bilimler
dc.subjectCell Biology
dc.subjectMolecular Biology
dc.subjectBiotechnology
dc.subjectApplied Microbiology and Biotechnology
dc.titleEffects of combined administration of doxorubicin and chloroquine on lung pathology in mice with solid Ehrlich ascites carcinoma
dc.typeMakale
dc.relation.journalBIOTECHNIC & HISTOCHEMISTRY
dc.contributor.departmentIstanbul Mehmet Akif Ersoy Thorac & Cardiovasc Su , ,
dc.contributor.firstauthorID3397231


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