dc.contributor.author | Sahin, Egemen | |
dc.contributor.author | Yilmaz, Zehra Kubra | |
dc.contributor.author | ÖZDEMİR, ÖZLEM | |
dc.contributor.author | ASLIM, BELMA | |
dc.contributor.author | SULUDERE, ZEKİYE | |
dc.date.accessioned | 2022-07-04T15:47:01Z | |
dc.date.available | 2022-07-04T15:47:01Z | |
dc.identifier.citation | Yilmaz Z. K. , ÖZDEMİR Ö., ASLIM B., SULUDERE Z., Sahin E., "A new bio-active asymmetric-Schiff base: synthesis and evaluation of calf thymus DNA interaction, topoisomerase II alpha inhibition, in vitro antiproliferative activity, SEM analysis and molecular docking studies", JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2022 | |
dc.identifier.issn | 0739-1102 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_c29e9cee-45c0-4557-882e-f1c5611d841b | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/184558 | |
dc.identifier.uri | https://doi.org/10.1080/07391102.2022.2039297 | |
dc.description.abstract | In this paper, the asymmetric-Schiff base 2-(4-(2-hydroxybenzylideneamino)benzylideneamino)benzoic acid (SB-2) was newly synthesized and characterized by various spectroscopic methods. The interaction of SB-2 with calf thymus DNA was investigated by UV-vis, fluorescence spectroscopy and molecular docking methods. It was determined that SB-2 effectively binds to DNA via the intercalation mode. DNA electrophoretic mobility experiments displayed that topoisomerase II alpha could not cleave pBR322 plasmid DNA in the presence of SB-2, confirming that the Schiff base acts as a topo II suppressor. In the molecular docking studies, SB-2 was found to show an affinity for both the DNA-topoisomerase II alpha complex and the DNA. In vitro antiproliferative activity of SB-2 was screened against HT-29 (colorectal) and HeLa (cervical) human tumor cell lines by MTT assay. SB-2 diminished the cell viability in a concentration- and incubation time-dependent manner. The ability of SB-2 to measure DNA damage in tumor cells was evaluated with cytokinesis-block micronucleus assay after incubation 24 h and 48 h. Light and scanning electron microscopy experiments of tumor cells demonstrated an incubation time-dependent increase in the proportion of apoptotic cells (nuclear condensation and apoptotic bodies) suggesting that autophagy and apoptosis play a role in the death of cells. Based on the obtained results, it may be considered that SB-2 is a candidate for DNA-targeting antitumor drug. Communicated by Ramaswamy H. Sarma | |
dc.language.iso | eng | |
dc.subject | Cancer Research | |
dc.subject | Molecular Biology | |
dc.subject | Drug Discovery | |
dc.subject | Aging | |
dc.subject | General Biochemistry, Genetics and Molecular Biology | |
dc.subject | Biochemistry | |
dc.subject | Biophysics | |
dc.subject | Structural Biology | |
dc.subject | Biochemistry (medical) | |
dc.subject | Life Sciences | |
dc.subject | Health Sciences | |
dc.subject | BİYOKİMYA VE MOLEKÜLER BİYOLOJİ | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | BİYOFİZİK | |
dc.subject | Biyoloji ve Biyokimya | |
dc.subject | Tıp | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Temel Tıp Bilimleri | |
dc.subject | Biyofizik | |
dc.subject | Biyokimya | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Sitogenetik | |
dc.subject | Temel Bilimler | |
dc.subject | Biochemistry, Genetics and Molecular Biology (miscellaneous) | |
dc.subject | Clinical Biochemistry | |
dc.title | A new bio-active asymmetric-Schiff base: synthesis and evaluation of calf thymus DNA interaction, topoisomerase II alpha inhibition, in vitro antiproliferative activity, SEM analysis and molecular docking studies | |
dc.type | Makale | |
dc.relation.journal | JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | |
dc.contributor.department | Gazi Üniversitesi , , | |
dc.contributor.firstauthorID | 3405600 | |