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dc.contributor.authorMehtiyev, Toghrul
dc.contributor.authorÖzden, Sibel
dc.contributor.authorKaraman, Ecem Fatma
dc.date.accessioned2023-02-21T08:49:54Z
dc.date.available2023-02-21T08:49:54Z
dc.date.issued2023
dc.identifier.citationMehtiyev T., Karaman E. F., Özden S., "Alterations in cell viability, reactive oxygen species production, and modulation of gene expression involved in mitogen–activated protein kinase/extracellular regulating kinase signaling pathway by glyphosate and its commercial formulation in hepatocellular carcinoma cells", Toxicology and Industrial Health, cilt.39, sa.2, ss.81-93, 2023
dc.identifier.issn0748-2337
dc.identifier.otherav_22ba2d5e-9ff6-4034-a4a8-18048ad83eb2
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/187004
dc.identifier.urihttps://doi.org/10.1177/07482337221149571
dc.description.abstract© The Author(s) 2023.Glyphosate (N-phosphonomethyl glycine) is a non-selective, organophosphate herbicide widely used in agriculture and forestry. We investigated the possible toxic effects of the glyphosate active compound and its commercial formulation (Roundup Star®) in the human hepatocellular carcinoma (HepG2) cell line, including their effects on the cytotoxicity, cell proliferation, reactive oxygen species (ROS) levels, and expression of oxidative stress-related genes such as HO-1, Hsp70 Nrf2, L-FABP, and Keap1. MTT and NRU tests indicated that the IC50 values of Roundup Star® were 219 and 140 μM, respectively, and because glyphosate failed to induce cell death at the studied concentrations, an IC50 value could not be determined for this cell line. Roundup Star at concentrations of 50 and 100 μM significantly increased (39.58% and 52%, respectively) cell proliferation, which 200 μM of glyphosate increased by 35.38%. ROS levels increased by 27.97% and 44.77% for 25 and 100 μM of Roundup Star and 32.74% and 38.63% for 100 and 200 μM of glyphosate exposure. In conclusion, Roundup Star and glyphosate significantly increased expression levels of selected genes related to the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling pathway. This suggests that ROS production and the MAPK/ERK signaling pathway may be key molecular mechanisms in the toxicity of glyphosate in liver cells.
dc.language.isoeng
dc.subjectFarmasötik Toksikoloji
dc.subjectSosyal ve Beşeri Bilimler
dc.subjectSosyoloji
dc.subjectYaşam Bilimleri
dc.subjectSağlık Bilimleri
dc.subjectTemel Bilimler
dc.subjectToksikoloji
dc.subjectHalk, Çevre ve İş Sağlığı
dc.subjectSağlık, Toksikoloji ve Mutajenez
dc.subjectFizik Bilimleri
dc.subjectEczacılık
dc.subjectMeslek Bilimleri
dc.subjectKAMU, ÇEVRE VE İŞ SAĞLIĞI
dc.subjectTOKSİKOLOJİ
dc.subjectSosyal Bilimler Genel
dc.subjectFarmakoloji ve Toksikoloji
dc.subjectSosyal Bilimler (SOC)
dc.subjectYaşam Bilimleri (LIFE)
dc.titleAlterations in cell viability, reactive oxygen species production, and modulation of gene expression involved in mitogen–activated protein kinase/extracellular regulating kinase signaling pathway by glyphosate and its commercial formulation in hepatocellular carcinoma cells
dc.typeMakale
dc.relation.journalToxicology and Industrial Health
dc.contributor.departmentİstanbul Üniversitesi , ,
dc.identifier.volume39
dc.identifier.issue2
dc.identifier.startpage81
dc.identifier.endpage93
dc.contributor.firstauthorID4248601


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