LAKE BEYSEHIR MICROBIAL POPULATION CHARACTERIZATION BY 16S METAGENOMICS STUDY

Abstract

Lake Beyşehir, located in the Central Anatolia Region, is the largest lake in the region, providing drinking water and irrigation. It is Turkey's third-largest lake and the largest freshwater lake. Although large populations use lakes in our country, there are not enough studies on prokaryotic diversity, so revealing the diversity is essential for lakes and the people around lakes. A metagenomic analysis allows for the determination of the species of microorganisms obtained through DNA isolation of sample directly taken from the environment without culturing, as well as the genetic structure and functional roles of these microorganisms and their impact on human and ecological health. We utilized NGS high-throughput methods for the metagenomic analysis of the Lake Beyşehir sequences of 16S rDNA (regions V3–V4). Illumina NovaSeq technology was used to sequence the variable regions. Metagenomic analysis was performed on these sequences using MOTHUR software, and prokaryotic diversity was explored, revealing the phyla Proteobacteria, Verrucomicrobia, Bacteroidetes, and Actino-bacteria.