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dc.contributor.authorOzturk, Şükrü
dc.contributor.authorSuer, İlknur
dc.contributor.authorCefle, Kıvanç
dc.contributor.authorPalanduz, Şükrü
dc.contributor.authorOzgur, Emre
dc.contributor.authorGezer, Uğur
dc.contributor.authorKaya, Murat
dc.contributor.authorCapik, Ozel
dc.contributor.authorKaratas, Omer Faruk
dc.date.accessioned2023-10-10T11:46:08Z
dc.date.available2023-10-10T11:46:08Z
dc.identifier.citationKaya M., Suer İ., Ozgur E., Capik O., Karatas O. F., Ozturk Ş., Gezer U., Palanduz Ş., Cefle K., "miR-145-5p suppresses cell proliferation by targeting <i>IGF1R </i>and <i>NRAS </i>genes in multiple myeloma cells", TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, 2023
dc.identifier.issn0250-4685
dc.identifier.othervv_1032021
dc.identifier.otherav_19ef53ad-5e31-47ad-b573-25aa6db446d5
dc.identifier.urihttp://hdl.handle.net/20.500.12627/189895
dc.identifier.urihttps://doi.org/10.1515/tjb-2023-0042
dc.description.abstractObjectives: Multiple myeloma (MM) is a common hematological cancer. Hence, it is important to conduct further studies investigating the molecular mechanisms in detail that contributes to myeloma genesis. In addition to genetic changes, epigenetic factors such as miRNAs may influence the expression of myeloma-related genes.Methods: Our study aimed to detect genes closely related to MM and miRNAs involved in the cancer process by changing the expression of these genes with bioinformatics tools and in vitro methods. Bioinformatics approaches identified hub miRNAs in our study that may have a role in the expression change of genes connected to myeloma. The functional impacts of the chosen miRNA on RPMI8226 and U266 cell lines and the effect of this miRNA on the expression changes of putative target genes were investigated.Results: The viability of miR-145-5p transfected cells was found to decrease compared to control cells and the expression of IGF1R and NRAS genes were found to be significantly suppressed in both cell lines at mRNA level. Decreased levels of the IGF1R and NRAS genes were confirmed in miR-145-5p transfected cells at the protein level as well as compared to control cells. In addition, IGF1R/miR-145-5p interaction was demonstrated via luciferase reporter assay. However, expression levels of EGFR, KLF4, IRS1, CDK4 and CDK6 candidate genes had no statistically significant difference in miR-145-5p transfected cells compared to control cells.Conclusions: Mir-145-5p was demonstrated to act as a tumor suppressor miRNA and inhibit the proliferation in MM cell lines via targeting IGF1R and NRAS.
dc.language.isoeng
dc.subjectYapısal Biyoloji
dc.subjectMoleküler Biyoloji
dc.subjectKlinik Biyokimya
dc.subjectKanser Araştırmaları
dc.subjectBiyokimya
dc.subjectYaşlanma
dc.subjectBiyokimya, Genetik ve Moleküler Biyoloji (çeşitli)
dc.subjectGenel Biyokimya, Genetik ve Moleküler Biyoloji
dc.subjectSitogenetik
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.subjectİlaç Keşfi
dc.subjectTemel Bilimler
dc.subjectYaşam Bilimleri
dc.titlemiR-145-5p suppresses cell proliferation by targeting <i>IGF1R </i>and <i>NRAS </i>genes in multiple myeloma cells
dc.typeMakale
dc.relation.journalTURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
dc.contributor.department, ,
dc.contributor.firstauthorID4403539


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