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dc.contributor.authorMekkey, Saleh D.
dc.contributor.authorEl-Bahy, Zeinhom M.
dc.contributor.authorKARAKUŞ, SELCAN
dc.contributor.authorNaz, Gul
dc.contributor.authorSalem, Mohamed A.
dc.contributor.authorAli Soomro, Razium
dc.contributor.authorPrasad Sharma, Bharat
dc.date.accessioned2023-10-10T13:14:23Z
dc.date.available2023-10-10T13:14:23Z
dc.identifier.citationNaz G., Salem M. A., Prasad Sharma B., Mekkey S. D., Ali Soomro R., KARAKUŞ S., El-Bahy Z. M., "Ti3C2Tx-Au hybrid composites-based electrochemical biosensors for calreticulin biomarker detection", Microchemical Journal, cilt.194, 2023
dc.identifier.issn0026-265X
dc.identifier.otherav_2eea1856-f7f1-4971-ae5a-34aa3c2959f0
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/190514
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85170714558&origin=inward
dc.identifier.urihttps://doi.org/10.1016/j.microc.2023.109307
dc.description.abstractA sensitive biosensor is critical for early breast cancer treatment and prognosis. Herein, a label-free electrochemical immunosensor is proposed for the sensitive detection of calreticulin (CALR), a new breast cancer biomarker. The biosensor relied upon an electroactive hybrid of ultra-thin Ti3C2Tx nanosheets preadsorbed with Au NPs and methylene blue (MB)(MB–Ti3C2Tx–Au), which served as redox-active centers and an electroactive probe to detect CALR biomarkers respectively. The detection mechanism followed a simple inhibition strategy, where the optimal differential pulse voltammetry (DPV) response of preadsorbed MB over the Ti3C2Tx–Au electrode decreased in proportion to the concentration of CALR biomarkers owing to the formation of the antibody-antigen immunocomplex. The biosensor could detect CALR-biomarker in the concentration range of 0.0015 to 0.94 ng mL−1 with a limit of detection (LOD) of 0.28 pg mL−1 and showed excellent antifouling properties against commonly encountered biomolecules such as hemoglobin (Ig), immunoglobulin G (IgG), neuron-specific enolase (NSE), and tumor necrosis factor-alpha (TNF). The proposed strategy provides an efficient method for utilizing MXene nanosheets to construct advanced biosensors with promising clinical applications.
dc.language.isoeng
dc.subjectKimya
dc.subjectKİMYA, ANALİTİK
dc.subjectSPEKTROSKOPİ
dc.subjectAnalitik Kimya
dc.subjectFizikokimya
dc.subjectSpektroskopi
dc.subjectTemel Bilimler (SCI)
dc.subjectFizik Bilimleri
dc.subjectTemel Bilimler
dc.titleTi3C2Tx-Au hybrid composites-based electrochemical biosensors for calreticulin biomarker detection
dc.typeMakale
dc.relation.journalMicrochemical Journal
dc.contributor.departmentMOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology , ,
dc.identifier.volume194
dc.contributor.firstauthorID4556656


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