The effects of naproxen sodium on specific parameters of L-strain cells over guided bone regeneration barrier materials

Abstract

There is strong evidence that nonsteroidal antiinflammatory drugs (NSAID) may exert a significant antiproliferative effect. This study evaluated the influence of NSAID on specific parameters of fibroblastic cells, in vitro, over two-guided bone regeneration (GBR) barrier materials. Fibroblast cells were cultured on bioabsorbable membrane made of collagen (Bio-Gide(R)- BG) and the most common nonresorbable membrane which is made of expanded polytetrafluoroethylene (ePTFE, Gore-Tex(R)- GT). Naproxen sodium (10 mM) was used as an analgesic drug. The fibroblast cells were cultured in vitro for 24 h and examined by scanning electron microscopy (SEM). Cells were cultured in the presence of H-3-thymidine to study cell proliferation. And also cell numbers and viabilities were measured. The difference between the means for each group were analyzed for statistical significance by Kruskal-Wallis one-way ANOVA followed by post hoc comparisons using the Dunn statistical method. Of all the six groups, the control group stimulated DNA synthesis more than the others. With respect to cell numbers, there was statistically significant difference between the control group and naproxen planted BG membrane group. The interpretation of our SEM images is that these two barriers and naproxen seem to have had the least effect on cellular morphology. These data suggest naproxen have an inhibitory effect on stimulation of DNA synthesis, cell numbers and viabilities. And also lacking adherence of cells to the membranes may be due to the physical properties of the materials.