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dc.contributor.authorKarlik, Elif
dc.contributor.authorUcarli, Cüneyt
dc.contributor.authorUzuner, Semian Karaer
dc.contributor.authorGurel, Filiz
dc.contributor.authorSHAHID, Muhammad Qasim
dc.date.accessioned2021-03-03T08:42:18Z
dc.date.available2021-03-03T08:42:18Z
dc.date.issued2015
dc.identifier.citationSHAHID M. Q. , Ucarli C., Karlik E., Uzuner S. K. , Gurel F., "SILENCING OF G-PROTEIN alpha-SUBUNIT IN BARLEY (Hordeum vulgare L.) BY VIGS AND ITS EFFECT ON THE PLANT GROWTH", TURKISH JOURNAL OF FIELD CROPS, cilt.20, sa.2, ss.259-264, 2015
dc.identifier.issn1301-1111
dc.identifier.otherav_18f66f27-faa6-4106-acf0-0f1a5928e69c
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/22096
dc.identifier.urihttps://doi.org/10.1757/tjfc.75064
dc.description.abstractG-proteins play important roles in a number of cellular and developmental processes in plants. In this study, we have cloned a 317-bp fragment encoding a part of barley G protein alpha-subunit into gamma-genome of barley stripe mosaic virus (BSMV). Barley seedlings were inoculated with this construct denoted as pSL038-1/G alpha at 3-leaf stage, and assayed for their growth, leaf area and membrane ion leakage. Silencing was achieved after 2 weeks of inoculation and confirmed by the decrease in G alpha subunit mRNAs in barley leaves. There was a significant decrease in plant growth in terms of plant height and leaf area following the viral infection. Leaf area in silenced plants were decreased by >10% compared to control plants (wild-type or inoculated by BSMV-PDS constructs). In conclusion, silencing resulted in etiolated phenotypes and growth retardation. Our optimized VIGS protocol in barley could be adopted to evaluate extensive physiological parameters and molecular changes in G alpha silenced plants.
dc.language.isoeng
dc.subjectToprak Verimliliği ve Gübreleme
dc.subjectToprak ve Bitki Besleme
dc.subjectZiraat
dc.subjectTarımsal Bilimler
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectTarım Bilimleri
dc.subjectTARIM BİLİMİ
dc.titleSILENCING OF G-PROTEIN alpha-SUBUNIT IN BARLEY (Hordeum vulgare L.) BY VIGS AND ITS EFFECT ON THE PLANT GROWTH
dc.typeMakale
dc.relation.journalTURKISH JOURNAL OF FIELD CROPS
dc.contributor.department, ,
dc.identifier.volume20
dc.identifier.issue2
dc.identifier.startpage259
dc.identifier.endpage264
dc.contributor.firstauthorID58079


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