Investigation of the Relationship Between Oxidative Stress and Glucose Signaling in Schizosaccharomyces pombe

Abstract

The invertase mutant defective in the glucose signaling pathway of () is resistant to glucose repression. This mutant is able to consume sucrose alongside glucose and grows in glucose-containing media with a generation time close to that of the wild type. Intracellular oxidation, protein carbonyl, and reduced glutathione levels and catalase, superoxide dismutase, and glutathione peroxidase activity were investigated in , to determine the relationship between oxidative stress response and glucose signaling. The expression profiles of some genes involved in regulation of glucose repression ( fructose-1,6-bis-phosphatase; hexokinase) and stress response ( and transcription factors; catalase; Cu,Zn superoxide dismutase) were analyzed using the quantitative real-time PCR technique. Oxidative stress response in seems to be affected by glucose signaling in a manner different from that caused by glucose deprivation.