K-carrageenan/PVA/nano-eggshell biocomposite-based non-enzymatic electrochemical biosensor for low-level urea detection

Abstract

As a comprehensive survey on both health and biosensor technologies, in this study, a non-enzymatic electrochemical biosensor based on kappa-carrageenan/PVA/nano-eggshell (K-carrageenan/PVA/nano-eggshell) biocomposite was prepared to detect low-level urea in phosphate-buffered solution (PBS). Novel K-carrageenan/PVA/nano-eggshell biocomposite was prepared by ultrasonics sonochemistry. The electrochemical biosensor showed a sensitivity of 0.018 mu A mu M-1 cm(-2)in a linear range of [250-1000] mu M urea. Urea detection limit of the biosensor was 60 mu M at room temperature.