dc.contributor.author | ÖKTEM, GÜLPERİ | |
dc.contributor.author | Bilir, Ayhan | |
dc.contributor.author | Korkmaz, Mevlut | |
dc.contributor.author | Zilan, Aydin | |
dc.contributor.author | Korkmaz, Seval | |
dc.contributor.author | Erguven, Mine | |
dc.date.accessioned | 2021-03-03T12:04:59Z | |
dc.date.available | 2021-03-03T12:04:59Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Bilir A., Erguven M., Zilan A., ÖKTEM G., Korkmaz S., Korkmaz M., "Evaluation and Comparison of In Vitro Biocompatibility of Poly (Glycolic Acid) and Poly (Lactide-Co-Glycolide Acid) on Mature Spheroids of Tumorigenic and Non-Tumorigenic Cell Lines", TURKIYE KLINIKLERI TIP BILIMLERI DERGISI, cilt.31, sa.1, ss.1-14, 2011 | |
dc.identifier.issn | 1300-0292 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_2bc3bab3-8df8-41e1-ae5c-c457282b0dc4 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/34177 | |
dc.identifier.uri | https://doi.org/10.5336/medsci.2009-16279 | |
dc.description.abstract | Objective: The aim of this study was to evaluate and compare in vitro biocompatibility and poly(glycolic acid) (PGA) and poly(lactide-co-glycolide acid) (PLGA) on tumorigenic and non-tumorigenic mature spheroids. Material and Methods: This is an in vitro experimental study. Tumorigenic (C6 glioma, SH-SY5Y, MDAH2774, MCF-7) and non-tumorigenic cells [CRL11372, primary osteoblasts (MCPO)] as well as their mature spheroids were cultured alone as a control group as well as in combination with PGA and PLGA. Total cell numbers, bromodeoxyuridine labeling index (BrDU-LI), apoptosis, morphology, and ultrastructure were evaluated. Results: PGA and PLGA significantly decreased the number of SH-SY5Y and C6 glioma cells; MDAH 2774 cells also decreased, but not significantly (p> 0.05). Low BrDU-LI (p< 0.05) with a high level of apoptosis (p< 0.05) at C6 glioma and a high level of BrDU-LI (p< 0.05) with a low level of apoptosis at MDAH2774 (p< 0.01) were noted. These biopolymers mostly decreased the number of CRL-11372 cells (p< 0.05), but indicated an increased apoptosis (p< 0.01) and significant BrDU-LI (p< 0.05). Biopolymers induced chromatin condensation (typical apoptotic ultrastructure) and vacuolization primarily at SH-SY5Y spheroids but rarely at MDAH-2774 spheroids. This apoptotic ultrastucture was most often observed at MCPO spheroids. PLGA and PGA induced similar BrDU-LI decreases among tumorigenic spheroids (p< 0.05), although this decrease was greater at MCF-7 (p< 0.05) in the PGA group. PGA primarily decreased BrDU-LI at CRL 11372 (p< 0.05), although the decrease was almost identical to that at MCPO for the two biopolymers (p< 0.05). A significant attachment affinity was determined at MDAH -2774 and C6 glioma spheroids. Conclusion: This study demonstrated the biocompatibility of PGA and PLGA at mature spheroids of tumorigenic and non-tumorigenic cell lines, which changed according to the cell type. | |
dc.language.iso | eng | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Temel Tıp Bilimleri | |
dc.subject | Tıp | |
dc.subject | Klinik Tıp (MED) | |
dc.subject | Klinik Tıp | |
dc.subject | TIP, GENEL & İÇECEK | |
dc.title | Evaluation and Comparison of In Vitro Biocompatibility of Poly (Glycolic Acid) and Poly (Lactide-Co-Glycolide Acid) on Mature Spheroids of Tumorigenic and Non-Tumorigenic Cell Lines | |
dc.type | Makale | |
dc.relation.journal | TURKIYE KLINIKLERI TIP BILIMLERI DERGISI | |
dc.contributor.department | Bezmiâlem Vakıf Üniversitesi , , | |
dc.identifier.volume | 31 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 1 | |
dc.identifier.endpage | 14 | |
dc.contributor.firstauthorID | 199390 | |