Population data of new 21 mini-InDels from Turkey

Abstract

Insertions/deletions (InDels) are a variety of polymorphisms that are observed with deletion or insertion or both, and are common in both somatic and gonosomal chromosomes. Since these loci have short amplicon lengths and have a high discrimination power when they are studied with 20-30 loci multiplexes, they are now being used as an alternative STR loci in forensic identification. In forensic sciences, sex chromosomes can be used according to the type and variety of the case. While the paternal kinship, and the relationship between father and son can be determined by using Y chromosome polymorphism, the relationship between father and daughter can be determined by the use of X chromosome polymorphism (Freitas et al., 2010; Manta et al. 2012; Pereira et al. 2009a, b; Pereira et al. 2012a, b; Martinez-Cortes et al. 2015; Weber et al., 2002). In this study, in order to provide information about the gene frequency of 21 gonosomal mini InDels panel, samples were obtained from 100 persons in representation of Turkish population. PCR and electrophoresis conditions were applied according to the protocol recommended by Szibor et al. (2005). Comparisons of gene frequencies and populations data of 21 loci were performed by Arlequin ver. 3.5.2.2. Powerstat (Promega) was used to determine the power of each locus in forensic identification. The mean heterozygosity ratio of 21 loci was determined as 0.400 and the discrimination power was defined as 99%. As a result of this study, since the gonosomal InDels multiplex, which consists of 21 loci, is polymorphic in consideration of the Turkish population and the discrimination power required for identification may be achieved, it may be used separately or together with currently available STR and SNP loci for solving cases.