The Therapeutic Effect of Autogenic Adipose Derived Stem Cells Combined with Autogenic Platelet Rich Plasma in Tendons Disorders in Horses in vitro and in vivo Research

Abstract

Naturally, occurring tendons injuries including superficial digital flexor tendinopathy are the most frequent musculoskeletal disorders in. performance horses. Conventional methods of treatment with non steroidal and steroidal anti-inflammatory medicaments in majority of cases lead to scar formation, reducing the quality and efficiency of tissue regeneration. Novel approach is aimed to use cells naturally present in an organism as regeneration enhancing factor. In conducted research, the intralesional injections of autologous adipose derived stem cells combined with autologous platelet concentrate therapeutic potential was investigated in horses with 8-12 weeks duration superficial digital flexor injury with severe scaring. Collected by clinical examinations data showed positive effects of autologous, adipose-derived mesenchymal stem cells combined with autologous platelet rich plasma injections on regeneration processes in the course of superficial flexor tendon injures in horses. On the basis of ultrasound examination, it was proved that the quality of healed tissue was significantly higher in experimental group, comparing to control group. Obtained results confirmed the beneficial pro-regeneration effects of stem cells/platelet concentrate combined injections. The obtained data may also serve as valuable source of information about morphology and behaviour of fat stem cells in culture or platelets appearance.