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dc.contributor.authorAltinkut, A
dc.contributor.authorGozukirmiz, N
dc.contributor.authorBajrovic, K
dc.contributor.authorGozukirmizi, N
dc.date.accessioned2021-03-03T16:13:52Z
dc.date.available2021-03-03T16:13:52Z
dc.identifier.citationAltinkut A., Gozukirmiz N., Bajrovic K., Gozukirmizi N., "High percentage of regeneration and transformation in chickpea", 3rd International ISHS Symposium on In Vitro Culture and Horticultural Breeding, JERUSALEM, İsrail, 16 - 21 Haziran 1996, ss.319-320
dc.identifier.othervv_1032021
dc.identifier.otherav_431eaa90-f316-4c83-9167-6a300a90c325
dc.identifier.urihttp://hdl.handle.net/20.500.12627/48830
dc.identifier.urihttps://doi.org/10.17660/actahortic.1997.447.63
dc.description.abstractEfficient tissue culture and gene transfer systems of Cicer arietinum L. cvs. Red chickpea, Canitez 87 and MB-10 varieties were established. The explants were taken from the shoot primordium part of the mature embryo. For callus induction MS+0.1 mg/l NAA+ 0.5 mg/l 2,4-D and for plant regeneration MS+0.2 mg/l IAA+ 0.5 mg/l Kn+ 0.2 mg/l 6-BAP containing media were used. Best regeneration response was for the MB-10 variety (85%). Young and wounded regenerated shoots were co-cultivated with A. tumefaciens 4404 and A. rhizogenes 9402 both containing pBI-121 plasmids. Explants cocultivated with A. tumefaciens produced crown gall and plantlets on selective media. However, applications with A. rhizogenes produced hairy roots. The existence of GUS and NPT II genes in the DNA isolated from plantlets and hairy roots was confirmed with Southern blot and digoxigenin labeling. The best transformation response was in Canitez 87 (12.7%) with A. tumefaciens and MB-10 variety (10.4%) with A. rhizogenes.
dc.language.isoeng
dc.subjectBİTKİ BİLİMLERİ
dc.subjectBitki ve Hayvan Bilimleri
dc.subjectBAHÇE BİTKİLERİ
dc.subjectTarımsal Bilimler
dc.subjectZiraat
dc.subjectBahçe Bitkileri
dc.subjectBitki Koruma
dc.subjectFitopatoloji
dc.subjectToprak ve Bitki Besleme
dc.subjectToprak Verimliliği ve Gübreleme
dc.subjectMikrobiyoloji
dc.subjectBiyoteknoloji
dc.subjectTemel Bilimler
dc.subjectYaşam Bilimleri
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectBİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectTarım Bilimleri
dc.subjectTARIM BİLİMİ
dc.titleHigh percentage of regeneration and transformation in chickpea
dc.typeBildiri
dc.contributor.department, ,
dc.contributor.firstauthorID118156


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