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dc.contributor.authorAkkoc, Tolga
dc.contributor.authorSagirkaya, Hakan
dc.contributor.authorCirit, Umut
dc.contributor.authorNak, Yavuz
dc.contributor.authorKoban, Evren
dc.contributor.authorBagis, Haydar
dc.contributor.authorNak, Deniz
dc.contributor.authorSenunver, Adem
dc.contributor.authorArat, Sezen
dc.contributor.authorKilicaslan, Ragip
dc.contributor.authorPabuccuoglu, SERHAT
dc.contributor.authorDemir, Kamber
dc.contributor.authorAslan, Ozgur
dc.contributor.authorTuna, Bilginer
dc.contributor.authorCetinkaya, Gaye
dc.contributor.authorDenizci, Melis
dc.contributor.authorCaputcu, Arzu T.
dc.date.accessioned2021-03-03T16:38:30Z
dc.date.available2021-03-03T16:38:30Z
dc.date.issued2011
dc.identifier.citationArat S., Caputcu A. T. , Akkoc T., Pabuccuoglu S., Sagirkaya H., Cirit U., Nak Y., Koban E., Bagis H., Demir K., et al., "Using cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattle", REPRODUCTION FERTILITY AND DEVELOPMENT, cilt.23, sa.8, ss.1012-1023, 2011
dc.identifier.issn1031-3613
dc.identifier.othervv_1032021
dc.identifier.otherav_454b6688-e378-434f-a18c-80bf9aba1b22
dc.identifier.urihttp://hdl.handle.net/20.500.12627/50256
dc.identifier.urihttps://doi.org/10.1071/rd11026
dc.description.abstractThe aim of this study was to clone native Anatolian Grey cattle by using different donor cell types, such as fibroblast, cartilage and granulosa cells cryopreserved in a gene bank and oocytes aspirated from ovaries of Holstein cows as the recipient cytoplasm source. One male calf from fibroblast, three female calves from granulosa cells and one female calf from cartilage cells were born healthy and at normal birthweights. No calves were lost after birth. The results demonstrated that the cloned calves had the same microsatellite alleles at 11 loci as their nuclear donors. However, the mtDNAs of the five Anatolian Grey cloned calves had different haplotypes from their donor cells and mtDNA heteroplasmy could not be detected in any of the clones. The birth of healthy clones suggests that the haplotype difference between the cell and oocyte donor did not affect the pre- or post-implantation development of the bovine nuclear transfer derived embryos in our study. The results showed that well established nuclear transfer protocols could be useful in conserving endangered species. In conclusion, somatic cell banking can be suggested as a tool in conservation programmes of animal genetic resources.
dc.language.isoeng
dc.subjectYaşam Bilimleri
dc.subjectGELİŞİMSEL BİYOLOJİ
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectÜREME BİYOLOJİSİ
dc.subjectBiyoloji ve Biyokimya
dc.subjectZOOLOJİ
dc.subjectBitki ve Hayvan Bilimleri
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectBiyokimya
dc.subjectTarımsal Bilimler
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectMikrobiyal Genetik
dc.subjectTemel Bilimler
dc.titleUsing cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattle
dc.typeMakale
dc.relation.journalREPRODUCTION FERTILITY AND DEVELOPMENT
dc.contributor.departmentBursa Uludağ Üniversitesi , ,
dc.identifier.volume23
dc.identifier.issue8
dc.identifier.startpage1012
dc.identifier.endpage1023
dc.contributor.firstauthorID43816


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