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Three-Dimensional Ingrowth of Bone Cells Within Biodegradable Cryogel Scaffolds in Bioreactors at Different Regimes

dc.contributor.authorGuzel, Elif
dc.contributor.authorBolgen, Nimet
dc.contributor.authorYang, Ying
dc.contributor.authorKorkusuz, Peter
dc.contributor.authorEl Haj, Alicia J.
dc.contributor.authorPiskin, Erhan
dc.date.accessioned2021-03-03T17:28:53Z
dc.date.available2021-03-03T17:28:53Z
dc.date.issued2008
dc.identifier.citationBolgen N., Yang Y., Korkusuz P., Guzel E., El Haj A. J. , Piskin E., "Three-Dimensional Ingrowth of Bone Cells Within Biodegradable Cryogel Scaffolds in Bioreactors at Different Regimes", TISSUE ENGINEERING PART A, cilt.14, sa.10, ss.1743-1750, 2008
dc.identifier.issn1937-3341
dc.identifier.otherav_49eb34c8-1fb1-4cba-82be-aaf42c214777
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/53131
dc.identifier.urihttps://doi.org/10.1089/ten.tea.2007.0277
dc.description.abstractThree-dimensional cell ingrowth within biodegradable cryogel scaffolds made of cross-linked 2-hydroxyethyl methacrylate (HEMA)-lactate-dextran with interconnected macropores was studied in bioreactors at different regimes (static, perfusion, and compression-perfusion). An osteoblast-like cell line (MG63) was used in these studies. The samples taken after selected times from the bioreactors were examined by microscopy techniques (light, SEM, TEM, and laser scanning confocal). The cell culture conditions were found to have a significant impact not only on the cell morphology, such as the extent of cell attachment and ingrowth, but also on cellular activities. Dynamic conditions (perfusion and/or compression) greatly improved cell ingrowth and extracellular matrix (ECM) synthesis. Alkaline phosphatase activity results confirmed the positive effect of dynamic conditions on bone cells.
dc.language.isoeng
dc.subjectTemel Bilimler
dc.subjectMühendislik ve Teknoloji
dc.subjectHÜCRE VE DOKU MÜHENDİSLİĞİ
dc.subjectBiyoloji ve Biyokimya
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectHÜCRE BİYOLOJİSİ
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectMÜHENDİSLİK, BİYOMEDİKSEL
dc.subjectMühendislik
dc.subjectMühendislik, Bilişim ve Teknoloji (ENG)
dc.subjectMALZEME BİLİMİ, BİYOMATERYAL
dc.subjectMalzeme Bilimi
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectBiyokimya
dc.subjectHistoloji-Embriyoloji
dc.subjectBiyomedikal Mühendisliği
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.titleThree-Dimensional Ingrowth of Bone Cells Within Biodegradable Cryogel Scaffolds in Bioreactors at Different Regimes
dc.typeMakale
dc.relation.journalTISSUE ENGINEERING PART A
dc.contributor.departmentHacettepe Üniversitesi , ,
dc.identifier.volume14
dc.identifier.issue10
dc.identifier.startpage1743
dc.identifier.endpage1750
dc.contributor.firstauthorID727399


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