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dc.contributor.authorDing, Zhen
dc.contributor.authorEkmekcioglu, Suhendan
dc.contributor.authorKim, Sun-Hee
dc.contributor.authorGrimm, Elizabeth
dc.contributor.authorAkcakaya, Handan
dc.contributor.authorDal Yontem, Fulya
dc.date.accessioned2021-03-03T17:43:16Z
dc.date.available2021-03-03T17:43:16Z
dc.date.issued2019
dc.identifier.citationDal Yontem F., Kim S., Ding Z., Grimm E., Ekmekcioglu S., Akcakaya H., "Mitochondrial dynamic alterations regulate melanoma cell progression", JOURNAL OF CELLULAR BIOCHEMISTRY, cilt.120, sa.2, ss.2098-2108, 2019
dc.identifier.issn0730-2312
dc.identifier.otherav_4b11e831-3de1-4dba-92ad-1d55c5fd48f4
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/53918
dc.identifier.urihttps://doi.org/10.1002/jcb.27518
dc.description.abstractResearch on mitochondrial fusion and fission (mitochondrial dynamics) has gained much attention in recent years, as it is important for understanding many biological processes, including the maintenance of mitochondrial functions, apoptosis, and cancer. The rate of mitochondrial biosynthesis and degradation can affect various aspects of tumor progression. However, the role of mitochondrial dynamics in melanoma progression remains controversial and requires a mechanistic understanding to target the altered metabolism of cancer cells. Therefore, in our study, we disrupted mitochondrial fission with mdivi-1, the reported inhibitor of dynamin related protein 1 (Drp1), and knocked down Drp1 and Mfn2 to evaluate the effects of mitochondrial dynamic alterations on melanoma cell progression. Our confocal study results showed that mitochondrial fission was inhibited both in mdivi-1 and in Drp1 knockdown cells and, in parallel, mitochondrial fusion was induced. We also found that mitochondrial fission inhibition by mdivi-1 induced cell death in melanoma cells. However, silencing Drp1 and Mfn2 did not affect cell viability, but enhanced melanoma cell migration. We further show that dysregulated mitochondrial fusion by Mfn2 knockdowns suppressed the oxygen consumption rate of melanoma cells. Together, our findings suggest that mitochondrial dynamic alterations regulate melanoma cell migration and progression.
dc.language.isoeng
dc.subjectHistoloji-Embriyoloji
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectSitogenetik
dc.subjectTemel Bilimler
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectTıp
dc.subjectHÜCRE BİYOLOJİSİ
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.titleMitochondrial dynamic alterations regulate melanoma cell progression
dc.typeMakale
dc.relation.journalJOURNAL OF CELLULAR BIOCHEMISTRY
dc.contributor.departmentUniversity of Texas System , ,
dc.identifier.volume120
dc.identifier.issue2
dc.identifier.startpage2098
dc.identifier.endpage2108
dc.contributor.firstauthorID262119


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