dc.contributor.author | Kazan, D | |
dc.contributor.author | Erarslan, A | |
dc.contributor.author | Ertan, H | |
dc.date.accessioned | 2021-03-03T19:49:55Z | |
dc.date.available | 2021-03-03T19:49:55Z | |
dc.date.issued | 1996 | |
dc.identifier.citation | Kazan D., Ertan H., Erarslan A., "Stabilization of penicillin G acylase against pH by chemical cross-linking", PROCESS BIOCHEMISTRY, cilt.31, sa.2, ss.135-140, 1996 | |
dc.identifier.issn | 1359-5113 | |
dc.identifier.other | av_569e5abb-fe30-44bc-a76f-adee47ec78e5 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/61144 | |
dc.identifier.uri | https://doi.org/10.1016/0032-9592(95)00038-0 | |
dc.description.abstract | The effect of pHs between 2.0 and 10.0 on the inactivation kinetics of penicillin G acylase (PGA) obtained from a mutant of Escherichia coli ATCC 11105 and the stabilization of enzyme against pH by chemical cross-linking with dimethyladipimidate (DMA) were studied. The inactivation mechanisms of native and DMA cross-linked PGA both appeared to obey first-order inactivation kinetics during prolonged incubation of enzyme solutions at 40 degrees C and at different pH values. The lowest inactivation rate constants were obtained with both native and DMA cross-linked PGA at pH 7.0. Inactivation rate constants decreased with an increase in pH from 2.0 to 7.0 and then increased again at pH values above 7.0. Chemical cross-linking of PGA by DMA resulted in the stabilization of enzyme against pH. Highest enhancement of pH stabilization (nearly four-fold) was obtained at pH 7.0 and 8.0. | |
dc.language.iso | eng | |
dc.subject | Kimya Mühendisliği ve Teknolojisi | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Biyoteknoloji | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Sitogenetik | |
dc.subject | Temel Bilimler | |
dc.subject | Mühendislik ve Teknoloji | |
dc.subject | Mühendislik | |
dc.subject | Mühendislik, Bilişim ve Teknoloji (ENG) | |
dc.subject | MÜHENDİSLİK, KİMYASAL | |
dc.subject | Mikrobiyoloji | |
dc.subject | BİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | BİYOKİMYA VE MOLEKÜLER BİYOLOJİ | |
dc.title | Stabilization of penicillin G acylase against pH by chemical cross-linking | |
dc.type | Makale | |
dc.relation.journal | PROCESS BIOCHEMISTRY | |
dc.contributor.department | , , | |
dc.identifier.volume | 31 | |
dc.identifier.issue | 2 | |
dc.identifier.startpage | 135 | |
dc.identifier.endpage | 140 | |
dc.contributor.firstauthorID | 117179 | |