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dc.contributor.authorBraidy, Nady
dc.contributor.authorLee, Matthew
dc.contributor.authorWong, Yie Kuan
dc.contributor.authorManefield, Michael
dc.contributor.authorMarquis, Christopher P.
dc.contributor.authorJugder, Bat-Erdene
dc.contributor.authorErtan, Haluk
dc.date.accessioned2021-03-04T07:41:00Z
dc.date.available2021-03-04T07:41:00Z
dc.date.issued2016
dc.identifier.citationJugder B., Ertan H., Wong Y. K. , Braidy N., Manefield M., Marquis C. P. , Lee M., "Genomic, transcriptomic and proteomic analyses of Dehalobacter UNSWDHB in response to chloroform", ENVIRONMENTAL MICROBIOLOGY REPORTS, cilt.8, sa.5, ss.814-824, 2016
dc.identifier.issn1758-2229
dc.identifier.otherav_5f5b708c-cfae-4467-be2d-0a1e3a73d498
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/66618
dc.identifier.urihttps://doi.org/10.1111/1758-2229.12444
dc.description.abstractOrganohalide respiring bacteria (ORB) are capable of utilising organohalides as electron acceptors for the generation of cellular energy and consequently play an important role in the turnover of natural and anthropogenically-derived organohalides. In this study, the response of a Dehalobacter sp. strain UNSWDHB to the addition of trichloromethane (TCM) after a 50 h period of its absence (suffocation) was evaluated from a transcriptomic and proteomic perspective. The up-regulation of TCM reductive dehalogenase genes (tmrABC) and their gene products (TmrABC) was confirmed at both transcriptional and proteomic levels. Other findings include the upregulation of various hydrogenases (membrane-associated Ni-Fe hydrogenase complexes and soluble Fe-Fe hydrogenases), formate dehydrogenases, complex I and a pyrophosphate-energized proton pump. The elevated expression of enzymes associated with carbon metabolism, including complete Wood Ljungdahl pathway, during TCM respiration raises interesting questions on possible fates of intracellular formate and its potential role in the physiology of this bacterium. Overall, the findings presented here provide a broader view on the bioenergetics and general physiology of Dehalobacter UNSWDHB cells actively respiring with TCM.
dc.language.isoeng
dc.subjectTemel Bilimler
dc.subjectMühendislik ve Teknoloji
dc.subjectTarımsal Bilimler
dc.subjectYaşam Bilimleri
dc.subjectÇevre Mühendisliği
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMikrobiyoloji
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectÇevre / Ekoloji
dc.subjectÇEVRE BİLİMLERİ
dc.titleGenomic, transcriptomic and proteomic analyses of Dehalobacter UNSWDHB in response to chloroform
dc.typeMakale
dc.relation.journalENVIRONMENTAL MICROBIOLOGY REPORTS
dc.contributor.departmentUniversity of New South Wales Sydney , ,
dc.identifier.volume8
dc.identifier.issue5
dc.identifier.startpage814
dc.identifier.endpage824
dc.contributor.firstauthorID235407


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