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dc.contributor.authorYILDIRIM, TUBA
dc.contributor.authorErturan, Zayre
dc.contributor.authorÇAYCI, YELİZ TANRIVERDİ
dc.contributor.authorDURUPINAR, BELMA
dc.contributor.authorBOZDOĞAN, BÜLENT
dc.contributor.authorÇOBAN, AHMET YILMAZ
dc.date.accessioned2021-03-04T08:10:26Z
dc.date.available2021-03-04T08:10:26Z
dc.date.issued2011
dc.identifier.citationÇOBAN A. Y. , ÇAYCI Y. T. , YILDIRIM T., Erturan Z., DURUPINAR B., BOZDOĞAN B., "Investigation of Plasmid-Mediated Quinolone Resistance in Pseudomonas aeruginosa Strains Isolated from Cystic Fibrosis Patients", MIKROBIYOLOJI BULTENI, cilt.45, sa.4, ss.602-608, 2011
dc.identifier.issn0374-9096
dc.identifier.othervv_1032021
dc.identifier.otherav_61f3decf-3557-415d-8a93-ab4530c691e6
dc.identifier.urihttp://hdl.handle.net/20.500.12627/68237
dc.description.abstractPseudomonas aeruginosa which is widely found in the environment, may lead to serious nosocomial infections. Due to its intrinsic resistance to many antibacterial agents, treatment of P.aeruginosa infections usually present difficulty. Quinolones, especially ciprofloxacin, are crutial antibiotics for the treatment of P.aeruginosa infections. However resistance developing to quinolones may become an important problem. Resistance to quinolones is often a result of chromosomal mutations and by the effect of efflux pumps. Recently plasmid-mediated quinolone resistance have been reportedin the members of Enterobacteriaceae family. The gene responsible for this resistance is called qnr. In addition to qnr genes there is also another gene called aac(6')-lb-cr responsible for plasmid-mediated quinolone resistance and aminoglycoside resistance. Limited studies which to screen P.aeruginosa strains for the presence of qnr gene region, revealed no positivity. The aim of this study was to investigate the plasmid-mediated quinolone resistance in P.aeruginosa strains isolated from cystic fibrosis patients. A total of 110 P.aeruginosa strains isolated from respiratory tract specimens from the patients were included in the study. Ciprofloxacin susceptibilities of the isolates were detected by Kirby-Bauer disk diffusion method according to CLSI guidelines. The presence of qnrA, qnrB, qnrC, qnrS and aac(6')-lb-cr genes were searched by multiplex polymerase chain reaction (PCR) with the use of specific individual primer pairs. As positive control strains, Escherichia coli J53 pMG252 (qnrA(1) positive), E.coli J53 pMG252 (qnrS(1) positive), E.coli J53 pMG258 (qnrB(1) and aac(6')-lb-cr positive), Klebsiella pneumoniae ref.15 (qnrB positive), Enterobacter cloacae ref.287 (qnrS positive), E.coli ref.20 (qnrA positive) and E.coli DH10 conjugated with pHS11 plasmid (qnrC positive) were used. Of 110 P.aeruginosa clinical isolates, 13 were found resistant to ciprofloxacin, while 7 were intermediate. However multiplex PCR yielded no positivity in terms of qnrA, qnrB, qnrC, qnrS and aac(6')-lb-cr gene regions. In conclusion, although our results indicated that none of the tested P.aeruginosa strains harboured those genes, further multicenter studies with large numbers of isolates are needed to confirm these results.
dc.language.isoeng
dc.subjectYaşam Bilimleri
dc.subjectMikrobiyoloji
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectTemel Bilimler
dc.titleInvestigation of Plasmid-Mediated Quinolone Resistance in Pseudomonas aeruginosa Strains Isolated from Cystic Fibrosis Patients
dc.typeMakale
dc.relation.journalMIKROBIYOLOJI BULTENI
dc.contributor.departmentOndokuz Mayıs Üniversitesi , ,
dc.identifier.volume45
dc.identifier.issue4
dc.identifier.startpage602
dc.identifier.endpage608
dc.contributor.firstauthorID202166


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